Production and characterization of a high molecular weight levan and fructo oligosaccharides from a rhizospheric isolate of Bacillus aryabhattai | [Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23 • 2023]

Author(s):

1. Anam Nasir: Industrial Biotechnology Division, NIBGE-C, PIEAS, Faisalabad, Pakistan

2. Fazal Sattar: Industrial Biotechnology Division, NIBGE-C, PIEAS, Faisalabad, Pakistan

3. Iram Ashfaq: Industrial Biotechnology Division, NIBGE-C, PIEAS, Faisalabad, Pakistan

4. Stephen R. Lindemann: Dept. of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN, 47907, USA

5. Ming-Hsu Chen: Dept. of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN, 47907, USA; School of Chemical and Biomedical Engineering, Nanyang Technological University, Block N1.2

6. Wim Vanden Ende: 62 Nanyang Drive, Singapore

7. Ebru ToksoyӦner: Laboratory of Molecular Plant Biology, KU Leuven, Leuven, Belgium

8. Onur Kirtel: Laboratory of Molecular Plant Biology, KU Leuven, Leuven, Belgium

9. Shazia Khaliq: Industrial Biotechnology Division, NIBGE-C, PIEAS, Faisalabad, Pakistan

10. M. Afzal Ghauri: Industrial Biotechnology Division, NIBGE-C, PIEAS, Faisalabad, Pakistan

11. Munir A. Anwar: Industrial Biotechnology Division, NIBGE-C, PIEAS, Faisalabad, Pakistan

Abstract:

Bacillus aryabhattai GYC2-3 strain, capable of synthesizing exopolysaccharide (EPS), was isolated from the rhizosphere of Taraxacum spp. plant. The EPS was identified as levan by 13C NMR spectroscopy and methylation analysis. The levan produced had a high weight average molecular weight of 5.317 × 107 Da and degree of branching 5.19%. Thinlayer chromatography and highperformance anion-exchange chromatography revealed that it is capable of producing broad range of fructooligosaccharides (FOS) and higher levels of FOS synthesis were observed with increasing sucrose concentrations. The optimum temperature, initial pH and sucrose concentration for levanbiosynthesis were studied to be 30 °C, 8.0 and 250 g/L, respectively. Levan production was increased by ~38%(26 g/L) when provided aeration as compare to static cultures. To the best of our knowledge, this is the first report on biosynthesis of levan and FOS by a B. aryabhattai strain.

Page(s): 148-148

DOI: DOI not available

Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023

Keywords:

Prebiotics , levan , Bacillus aryabhattai , Fructooligosaccharides , Exopolysaccharide

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