Vitamins C and E Ameliorate the Sodium Arsenate Induced Interstitial Fibrosis and Tubular Atrophy in Fetal Kidneys of Albino Mice. | [Biomedica • 2017]

Author(s):

1. Khalid Parvez Lone: Professor of Physiology and Acting Head of Anatomy Department, Institute: University of Health Sciences, Khayaban-e-Jamia Punjab, Lahore, Pakistan.

2. F Qureshi: Anatomy Department, Institute: University of Health Sciences, Khayaban-e-Jamia Punjab, Lahore, Pakistan.

3. M Tahir: Department of Anatomy, University of Health Sciences, Lahore – Pakistan

Abstract:

Objectives: Epidemiological evidence indicates that exposure to arsenic contaminated drinking water during pregnancy may lead to various congenital abnormalities. The fetal renal tissue is impaired in relation to the developmental stage of kidney and to the amount and duration of arsenic exposure. This study evaluated the role of vitamin C and E in preventing the sodium arsenate induced mutilation of developing renal tissue of albino mice. Materials & Methods: Albino female mice of BALB/c strain (24), were randomly divided into 4 groups after confirmation of pregnancy. The control group A1 received 0.1 ml/kg body weight distilled water; group A2 was given a single dose of sodium arsenate (35 mg/kg) body weight on 8th gestational day (GD) by intra-peritoneal (I/P) injection. The groups A3 and A4 were treated with sodium arsenate (35 mg/kg) body weight on 8th GD and vitamin C, (9mg/kg/day) and vitamin E (15 mg/kg/day) by I/P injections, from 8th GD for the rest of the pregnancy period. The fetal kidneys were extracted on 18th GD. Three coronal sections stained with PAS and Masson's trichrome from each kidney, were randomly chosen and examined for tubular injury and interstitial fibrosis. Results: The mean scoring for histological changes in fetal kidney tubules/10 fields among various study groups was statistically significant (p < 0.000). Group A2 showed severe tubular necrosis and interstitial fibrosis confirmed by Masson's trichrome staining. Group A3 (vit C) and A4 (vit E) depicted mild to moderate improvement in tubular necrosis and interstial fibrosis. Conclusions: This study supports the role of vitamin C and E in ameliorating the developing renal tissue from the oxidative stress induced by sodium arsenate.

Page(s): 284-290

DOI: DOI not available

Published: Journal: Biomedica, Volume: 33, Issue: 4, Year: 2017

Keywords:

Keywords are not available for this article.

References:

[1] DolkH,VrijheidM.,,2003,The impact of environmental pollution on congenital anomalies,British Medical Bulletin,1,25-45

[2] SoderlandP,LovekarS,WeinerDE,BrooksDR,KaufmanJS,,,Chronic kidney disease associated with environmental toxins and exposures,2010 May,31,254-64

[3] JomovaK,JenisovaZ,FeszterovaM,BarosS,LiskaJ,HudecovaD,RhodesCJ,ValkoM.,Arsenic,,2011,: toxicity, oxidative stress and human disease,Journal of Applied Toxicology,1,95-107

[4] FangYZ,YangS,WuG,,,Free radicals, antioxidants, and nutrition,Nutrition,31,872-9

[5] LemleyKV,KRIzWI,,,Anatomy of the renal interstitium,1991 Mar,1,370-81

[6] KaisslingB,Le HirM. Characterization,,,and distribution of interstitial cell types in the renal cortex of rats,1994 Mar,1,709-20

[7] FukagawaM,NodaM,ShimizuT,KurokawaK.,,1999,Chronic progressive interstitial fibrosis in renal disease-are there novel pharmacological approaches?,Nephrology Dialysis Transplantation,1,2793-5

[8] LiuJ,LiuY,HabeebuSM,WaalkesMP,KlaassenCD,,2000,Chronic combined exposure to cadmium and arsenic exacerbates nephrotoxicity, particularly in metallothionein-I/II null mice,Toxicology,5,157-66

[9] ChuW,LiC,QuX,ZhaoD,WangX,YuX,CaiF,LiangH,ZhangY,ZhaoX,LiB,,,Arsenic-induced interstitial myocardial fibrosis reveals a new insight into drug-induced long QT syndrome,Cardiovascular research,1,90-8

[10] FerzandR,GadahiJA,SalehaS,AliQ,,2008,Histological and haematological disturbance caused by arsenic toxicity in mice model,Pakistan journal of biological sciences: PJBS,11,1405-13

[11] RamanathanK,BalakumarBS,PanneerselvamC.,,,Effects of ascorbic acid and a-tocopherol on arsenic-induced oxidative stress,Human & experimental toxicology,21,675-80

[12] KaliaK,FloraSJ,,2005,Strategies for safe and effective therapeutic measures for chronic arsenic and lead poisoning,Journal of occupational health,47,1-21

[13] BanerjeeP,BhattacharyyaSS,BhattacharjeeN,PathakS,BoujedainiN,BelonP,Khuda-BukhshAR,,,Ascorbic acid combats arsenic-induced oxidative stress in mice liver,2009 Feb,28,639-49

[14] PanX,DaiY,LiX,NiuN,LiW,LiuF,ZhaoY,Yu Z,,,Inhibition of arsenic induced-rat liver injury by grape seed exact through suppression of NADPH oxidase and TGFβ/S mad activation,2011 Aug,1,323-31

[15] UmarBU,,2007,Effect of hexane extract of spinach in the removal of arsenic from rat,Bangladesh Journal of Pharmacology,2,27-34

[16] StumpDG,HolsonJF,FleemanTL,NemecMD,FarrCH,,1999,Comparative effects of single intraperitoneal or oral doses of sodium arsenate or arsenic trioxide during in utero development,Teratology,1,283-91

[17] SinghAP,GoelRK,KaurT.,,,Mechanisms pertaining to arsenic toxicity,,18,87-

[18] HughesMF,,,Arsenic toxicity and potential mechanisms of action,2002 Jul,7,1-6

[19] RanaSV,,,Protective effect of ascorbic acid against oxidative stress induced by inorganic arsenic in liver and kidney of rat.,,,-

[20] Al-AttarAM,,,Antioxidanteffect of vitamin E treatment on some heavy metals-induced renal and testicular injuries in male mice,Saudi journal of biological sciences, 2011 Jan,31,63-72

[21] AldanaL,TsutsumiV,CraigmillA,SilveiraMI,,2001,α-Tocopherol modulates liver toxicity of the pyrethroidcypermethrin,Toxicology letters,30,107-16

[22] JohnS,KaleM,RathoreN,BhatnagarD.,,,Protective effect of vitamin E in dimethoate and malathion induced oxidative stress in rat erythrocytes,2001 Sep,30,500-4

[23] BirriPN,PérezRD,CremonezziD,PérezCA,RubioM,BongiovanniGA,,,Association between As and Cu renal cortex accumulation and physiological an histological alterations after chronic arsenic intake,Environmental research,31,417-23

[24] ChinoyNJ,ShahSD,,2004,Beneficial effects of some antidotes in fluoride and arsenic induced toxicity in kidney of mice,Fluoride,1,151-61

[25] MazumderDG,,,Effect of drinking arsenic contaminated water in children,2007 Dec,1,925-

[26] SinghN,KumarD,LalK,RaisuddinS,SahuAP,,,Adverse health effects due to arsenic exposure: modification by dietary supplementation of jaggery in mice,2010 Feb,1,247-55

[27] YuM,XueJ,LiY,ZhangW,MaD,LiuL,,,Resveratrol protects against arsenic trioxide-induced nephrotoxicity by facilitating arsenic metabolism and decreesing oxidative stress,2013 Jun,1,1025-35

Citations

Downloads

Views