Author(s):
1. Aymen Ajmal:
Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan
2. Yusra Jamil:
Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan
3. Jalwa Ahmad:
Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan
4. Amjad Iqbal:
Department of Food Science & Technology, Abdul Wali Khan University Mardan, Pakistan
5. Ayaz Ahmad:
Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan
Abstract:
Diabetes mellitus is termed as a progressive human metabolic disorder, primarily characterized by hyperglycemia. Current pharmacological measures to manage diabetes and it adverse effects pushed the scientific community to search for natural, yet novel, potent and cost-effective drugs. The seeds and pods of Abelmoschus esculentus commonly known as okra were soaked in water to collect a mucilage. The mucilage was further fractionated through Bio-GelP10and each fraction was tested to establish their antidiabetic potential. The okra mucilage was obtained by soaking the okra pods and seeds in distilled water and the collected mucilage was fractioned by using gel permeation chromatography. The biochemical analysis exhibited the presence of pentoses, hexoses, saturated and unsaturated uronic acids. In vitro antidiabetic activity of selected fractions was carried out using a-amylase, a-glucosidase and yeast glucose uptake assay. Fraction O24 significantly (P=0.05) inhibited the a-amylase activity (IC50 was 265.75 µg/mL). The fraction O32 on the other hand significantly (P=0.05) inhibited the a-glucosidase (IC50 was 441.87 µg/mL). Likewise, the fraction O36 significantly (P=0.05) reduced the yeast glucose uptake. In vivo antidiabetic activity of Balb/C mice was monitored after inducing the diabetes by injecting the mice with 1% alloxan. Blood glucose levels along with the weight of experimental mice were recorded for a period of 14 days after injection. Each fraction of the okra mucilage at three different concentrations (100 mg/kg, 200 mg/kg, 400 mg/Kg body weight) were screened for in vivo antidiabetic activity. Results revealed that the fraction OK1(400 mg/Kg body weight) reduced the blood glucose level of alloxan-induced diabetic mice by 74.85%. Similarly, the alloxan-induced diabetic mice group treated with fraction Ok3 resulted in weight gain and at day-14, the recorded average weight was 23.90 g, which was comparable to a group treated with standard drug. The findings of this study conclude that okra pods and seeds mucilage comprise of bioactive polysaccharides fraction that exhibited promising anti-diabetic activity.
Page(s):
8-8
DOI:
DOI not available
Published:
Journal: International Food & Nutrition Conference" August 18th-20th, 2022, University of Swat, Swat (Book of Abstracts), Volume: 1, Issue: 1, Year: 2022
Keywords:
Diabetes mellitus
,
Abelmoschus esculentus
,
Diabetes mellitus
,
Blood glucose level
,
Polysaccharides
,
alpha amylase
References:
References are not available for this document.
Citations
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