Transcriptome Survey Reveals Candidate Genes Involved in Lipid Metabolism of Carya Illinoinensis. | [International Journal of Agriculture and Biology • 2018]

Author(s):

1. Xiaodong Jia: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China

2. Mingzhi Li: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China

3. Huiting Luo: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China

4. Min Zhai: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China

5. Zhongren Guo: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China

6. Yongrong Li: Nanjing Green Universe Pecan Scientific Company, Nanjing 210007, China

7. Yushan Qiao: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

8. Liangju Wang: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

Abstract:

Pecan [Carya illinoinensis (Wangenh.) K. Koch] kernel contains high proportion of fatty acid, which has the benefits of reducing the risk of blood and heart diseases. However, the molecular mechanism of lipid metabolism in pecan kernels has remained unclear. In this study, the changes of fatty acids in developing pecan kernels (cultivar Pawnee) was monitored using gas chromatography (GC) method, and the transcriptome analysis was performed on four critical stages of lipid accumulation. Twenty-five fatty acids were identified and a total of 73,262 unigenes were obtained after assembling, in which 7709 unigenes were annotated in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The top annotated pathway within “Metabolism” catalog in KEGG was “Carbohydrate metabolism”. A putative pecan lipid metabolic pathway was reconstructed including 26 homologs consist of 93 unigenes. The stearoyl-ACP desaturase (SAD) and the omega-6 fatty acid desaturase (FAD2) were found play vital roles in the formation of the high oleic acid content and corresponding fatty acid ratio in pecan kernel. The acetyl coenzyme A carboxylase (ACCase), the glycerol-3-phosphate acyltransferase (GPAT), the diacylglycerol acyltransferase (DGAT), and the phospholipid: diacylglycerol acyltransferase (PDAT) were found may play important roles in pecan lipid accumulation too. Transcription factor (TF) families like AP2-EREBP, bZIP, ARF, and C3H might have positive transcriptional regulations to lipid biosynthesis in pecan, while MYB might have negative transcriptional regulations. To our knowledge, this is the first report of global transcriptional profile of pecan kernels. These data will provide a theoretical basis for future genetic modification of fatty acids in pecan and other oilseed.

Page(s): 991-1004

DOI: 10.17957/IJAB/15.0591

Published: Journal: International Journal of Agriculture and Biology, Volume: 20, Issue: 5, Year: 2018

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