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Acute and chronic impact of smoking on salivary and serum total antioxidant capacity.
Author(s):
1. Huseyin Kurku: Department of Biochemistry, Konya Training and Research Hospital, Konya
2. Murat Kacmaz: Department of Medical Biochemistry, Faculty of Medicine, Kirikkale University, Kirikkale, Turkey
3. Ucler Kisa: Department of Medical Biochemistry, Faculty of Medicine, Kirikkale University, Kirikkale, Turkey
4. Ozlem Dogan: Mardin State Hospital, Unit of Biochemistry, Mardin, Turkey
5. Osman Caglayan: Department of Medical Biochemistry, Faculty of Medicine, Kirikkale University, Kirikkale, Turkey
Abstract:
Objective: To investigate increased oxidative stress in saliva of smokers along with their serum. Methods: The case-control study was conducted from September to December 2008 in the Department of Biochemistry of the Medical School, Kirikkale University, Kirikkale, Turkey. A blood sample and saliva samples before and after smoking were collected from the smokers, while blood and saliva samples were taken from the controls. All samples were taken concurrently. The samples were measured for total antioxidant capacity, total oxidant stress, oxidative stress index, malondialdehyde, nitric oxide and total sulfhydryl groups. Levels of superoxide dismutase and glutathione peroxidase were also measured on saliva samples. SPSS 13 was used for statistical analysis. Results: Of the 54 subjects in the study, 27(50%) were smokers with a mean age of 28.4±5.42 years, and 27(50%) were controls with a mean age of 29.7±8.03 years. Total oxidant stress, oxidative stress index, malondialdehyde and nitric oxide levels were found higher in the serum samples of smokers (p
Page(s): 164-169
DOI: DOI not available
Published: Journal: Journal of Pakistan Medical Association, Volume: 65, Issue: 2, Year: 2015
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