Transcriptional Regulation of Spermatogenesis- Related Gene CDK16 in Banna Mini-Pig Inbred Line | [Pakistan Journal of Zoology • 2025]

Author(s):

1. Hongmei Dai: College of Animal Science and Technology, Yunnan Agricultural University,Kunming, Yunnan,China

2. Shixiong Xu: College of Animal Science and Technology, Yunnan Agricultural University,Kunming, Yunnan,China

3. Zhipeng Liu: College of Animal Science and Technology, Yunnan Agricultural University,Kunming, Yunnan,China

4. Hailong Huo: Yunnan Open University,Kunming, Yunnan,China

5. Fuhua Yang: College of Animal Science and Technology, Yunnan Agricultural University,Kunming, Yunnan,China

6. Xia Zhang: College of Life Science, Lyuliang University,Lvliang, Shanxi,China

7. Jinlong Huo: College of Animal Science and Technology, Yunnan Agricultural University,Kunming, unnan, China; Department of Biology, University of Rochester,Rochester, New York,USA

Abstract:

CDK16 is known to have significant involvement in various biological processes of mammalian spermatogenesis. Here, the whole-transcriptome sequencing was performed on the testes of 12-monthold adult boars of Banna mini-pig inbred line (BMI), which identified the CDK16 gene as being highly expressed in the BMI testes. The initial average expression of the CDK16 gene in BMI testes was found to be 4,385, with a corrective average expression value (TPM) of the corresponding transcript ENSSSCT00000035953.3 being 54.3489. The full-length coding region of CDK16 obtained from BMI testes was 1,509 bp (GenBank accession number: OP094608). Gene structure analysis revealed that the CDK16 gene was located on chromosome X of the pig genome and comprised 16 exons. Additionally, Protein structure analysis showed that CDK16 consisted of 502 amino acids with a conserved S_TKc domain. Phylogenetic analysis indicated that the amino acid sequence of CDK16 was highly conserved across mammalian species in evolution. PPI network, KEGG and GO analyses suggested that CDK16 interacted with 50 proteins, with mainly involving in the cell cycle, cellular senescence, p53 signaling pathway, protein kinase activity, phosphorylation, and G1/S transition of mitotic cell cycle. Furthermore, correlation analysis between these proteins and RNA-seq data from BMI testes revealed that CDK16 was significantly associated with CDC6, CDKN1A, BARD1, CCNF, ACTR1B, CDKN1B, CKS2, CCND1, and CCNY. Functional annotations indicated that CDK16 was mainly involved in 9 GO terms, including four molecular functions (MF), three cellular components (CC), and two biological processes (BP). Furthermore, the ceRNA network analysis showed that BMI CDK16 was primarily regulated by six miRNAs including ssc-miR-21-3p, ssc-miR-296-3p, ssc-miR-296-5p, ssc-miR-370, ssc-miR-490 and ssc-miR-532-3p. Overall, our findings revealed the crucial role of CDK16 in BMI spermatogenesis, representing a promising candidate for further investigation.

Page(s): 517-529

DOI: 10.17582/journal.pjz/20230609020618

Published: Journal: Pakistan Journal of Zoology, Volume: 57, Issue: 2, Year: 2025

Keywords:

Functional annotation , Banna minipig inbred line BMI , Wholetranscriptome sequencing , CDK16 , Transcriptional regulatory

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