The Combined Efect of Mentha spicata, Perilla frutescens, and Ocimum basilicum Essential Oils with Norfloxacin Against Antibiotic-Resistant Escherichia coli Responsible for Pyometra in Dogs | [Journal of animal health and production • 2025]

Author(s):

1. Nguyen Van Vui: Animal Science and Veterinary Medicine Department, Agriculture and Aquaculture Faculty, Tra Vinh University, Tra Vinh City, Vietnam

2. Nguyen Van Tung Lam: Department of Assessment, Tra Vinh University, Vietnam.

3. Nguyen Thuy Linh: Animal Science and Veterinary Medicine Department, Agriculture and Aquaculture Faculty, Tra Vinh University, Tra Vinh City, Vietnam

4. Le My Duyen: Animal Science and Veterinary Medicine Department, Agriculture and Aquaculture Faculty, Tra Vinh University, Tra Vinh City, Vietnam

Abstract:

Pyometra is a serious reproductive condition in female dogs, often caused by antibiotic-resistant Escherichia coli, which complicates treatment due to its resistance to common antibiotics. This study explores the potential of essential oils from Mentha spicata, Perilla frutescens, and Ocimum basilicum to combat Escherichia coli strains isolated from pyometra cases, both alone and in combination with the antibiotic norfloxacin. Using the disk difusion method, we assessed the antibiotic resistance profiles of the bacteria, revealing significant resistance to amoxicillin, ampicillin, ceftazidime, streptomycin, colistin, and doxycycline. The bactericidal activity of the essential oils was determined through minimum bactericidal concentration (MBC) tests, with Mentha spicata exhibiting the highest MBC, significantly outperformed by norfloxacin. Notably, Ocimum basilicum and Mentha spicata demonstrated synergistic and additive efects with norfloxacin, while Perilla frutescens had no significant interaction. This study highlights the promising role of essential oils as adjuncts to antibiotics in treating antibiotic-resistant Escherichia coli infections in dogs, providing new insights into potential therapeutic strategies for pyometra.

Page(s): 377-386

DOI: 10.17582/journal.jahp/2025/13.2.377.386

Published: Journal: Journal of animal health and production, Volume: 13, Issue: 2, Year: 2025

Keywords:

Antibacterial , Escherichia coli , Essential oils , synergistic , Pyometra , AntibioticResistance

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