The detection of braf-v600e mutation in hairy cell leukaemia by polymerase chain reaction and study of its associated clinicohaematological parameters | [Pakistan Armed Forces Medical Journal • 2022]

Author(s):

1. Muhammad Ijaz Iqbal: Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi, Pakistan

2. Helen Mary Robert: Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi, Pakistan

3. Nabeela Khan: Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi, Pakistan

4. Rafia Mahmood: Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi, Pakistan

5. Ayesha Khurshid: Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi, Pakistan

6. Saleem Ahmed Khan: Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi, Pakistan

Abstract:

Objective: To contemplate the occurrence of a rare mutation (BRAF V600E) in the patients having Hairy cell leukemia in our setting, which wouldserve asuseful analytic and diagnostic criteria. Study Design: Analytical cross sectional study. Place and Duration of Study: Armed Forces Institute of Pathology Rawalpindi Pakistan, from Oct 2017 to Oct 2018. Methodology:A sample of 36 patients with a suspicion of Hairy cell leukemia were included in this investigation. Complete blood counts, bone marrow examination (trephine biopsy and aspiration), cyto-chemistry and staining of the patients suspected to have Hairy cell leukemia was performed. PCR was done for detection of BRAF V600E mutation. Results: 29 (80.5%) cases showed a positive BRAF mutational analysis and 7 (19.4%) cases showed a negative result. Conclusion:Almost all the cases of classic Hairy cell leukemia had BRAF V600 E mutation.Isolating this mutation can be used as a regular tool in detection of disease and monitoring disease progression and relapse.

Page(s): 178-181

DOI: 10.51253/pafmj.v72iSUPPL-2.3083

Published: Journal: Pakistan Armed Forces Medical Journal, Volume: 72, Issue: S2, Year: 2022

Keywords:

complete blood counts , BRAF V600E mutation , Bcell lymphoproliferative , Chronic lymphocytic leukemia , Monocytopenia , Pallor

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