kinetic studies of proteases from bacillus sp. | [Pakistan Journal of Life and Social Sciences • 2019]

Author(s):

1. Kashif Younas Butt: Department of Chemistry, Government Murray College, Sialkot, Pakistan

2. Muhammad Ishfaq Ghori: Department of Chemistry, Government Murray College, Sialkot, Pakistan

3. Khuda Bukhsh Saleemi: Punjab Medical College, University of Health Sciences, Lahore, Pakistan

4. Syeda Rubina Gilani: Department of Chemistry, University of Engineering and Technology, Lahore, Pakistan

5. Awais Altaf: Department of Biochemistry, Government College University, Faisalabad, Pakistan

Abstract:

Proteases have essential qualities which have attained the attention of researches from different domains including biotechnology and have thus become important group of enzymes both for industrial use and academic research purpose. Bacillus sp. is commonly used for isolation and extraction of protease enzyme. This study reports the kinetics of proteases from Bacillus sp. Results showed that maximum/optimum activity was recorded at 60°C temperature, 7.5 pH and 48 hours of continuous shaking at 220 rpm. The effects of different metal-ions on activity of proteases was also assessed. Lower value of KM=0.2 obtained from LineweaverBurk plot for the substrate demonstrated good association/relationship between substrate and protease enzyme. Dependence of the enzyme activity on the concentration of substrate revealed a good agreement between the experimental and theoretical values. PMSF inhibition proved that the proteases produced were serine proteases. Overall, the unique characters of proteases isolated from the Bacillus sp. suggested this enzyme for having remarkable potential for use in different industrial applications.

Page(s): 111-115

DOI: DOI not available

Published: Journal: Pakistan Journal of Life and Social Sciences, Volume: 17, Issue: 2, Year: 2019

Keywords:

Bacillus sp , Kinetic study Proteases

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