A validated RP-HPLC method for the determination of piperidone analogue of curcumin | [Pakistan Journal of Pharmaceutical Sciences • 2020]

Author(s):

1. Abdullah Alomrani: Department of Pharmaceutics, College of Pharmacy, King Saud University,Riyadh,Saudi Arabia

2. Samiah Alhabardi: Department of Pharmaceutics, College of Pharmacy, King Saud University,Riyadh,Saudi Arabia

3. Abdelilah Aboussekhra: Department of Molecular Oncology, King Fasial Specialist Hospital and Research Center,KSA,

4. Aws Alshamsan: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia

5. Mohamed I Attia: Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia

6. Bushra Al Quadeib: Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia

Abstract:

Curcumin (Diferuloylmethane) is a natural product extracted from the root of Curcuma longa. 5-Bis (4hydroxy-3-methoxybenzylidene)-N-methyl-4-piperidone, the piperidone analogue of curcumin (PAC), was one of the analogues that, demonstrated potential anticancer effects against breast and colon cancers compared with native curcumin. A simple, accurate, and rapid isocratic reverse phase high performance liquid chromatography (HPLC) analytical method utilizing UV detection was developed and validated for the determination of PAC utilizing C18 column with run time was 7 min. Chromatogram showed a peak of PAC at retention time of 5.8±0.92 min. The method was validated for linearity, accuracy, precision, limit of detection, limit of quantitation and robustness. Linear relationship (r > 0.99) was observed between AUP of PAC and the corresponding concentrations over 100-10000µg/mL. The LOQ of this assay was 3.9ng/mL with a corresponding relative standard deviation of 4.8 and 4.0%. The LOD was 13.1ng/mL at a signal-to-noise ratio of >3.

Page(s): 685-694

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 33, Issue: 2, Year: 2020

Keywords:

Piperidone analogue of curcumin

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