Virtual screening and biological evaluation of avian erythroblatosis virus e26 oncogene (ets-1) inhibitor. | [Journal of Animal and Plant sciences • 2014]

Author(s):

1. S. Mehreen: Department of Theriogenology, University of Veterinary and Animal Sciences, Lahore, Pakistan

2. A. Javeed: Department of Theriogenology, University of Veterinary and Animal Sciences, Lahore, Pakistan

3. M. Ashraf: Department of Theriogenology, University of Veterinary and Animal Sciences, Lahore, Pakistan

4. A. Riaz: Department of Theriogenology, University of Veterinary and Animal Sciences, Lahore, Pakistan

5. M. Hussain: Department of Pharmacology & Toxicology, University of Veterinary and Animal Sciences, Lahore, Pakistan

6. L. Shakir: Department of Pharmacology & Toxicology, University of Veterinary and Animal Sciences, Lahore, Pakistan

Abstract:

This study was designed to identify and characterize novel inhibitor of Ets-1 by using virtual screening approach combined with biological evaluation of anti-angiogenic ability of compound. Virtual screening of Indian plant anticancer compound database was performed against phosphorylation site (Threonine-38) of Ets-1 by Auto Dock 4.02. A compound with lowest energy score (-4.28kcal/mol) was selected. Biological activity of compound obtained from virtual screening was further evaluated by performing chick chorioallantoic membrane (CAM) assay. Different concentrations (0.5 %, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 6%, and 12%) of aqueous extract of saffron plant containing screened compound (Picrocrocin) were applied to CAMs and scanning probe image processor was used for quantification of membrane vasculature. Results showed that application of 2% and 3% concentration of extract showed reduction in diameter of blood vessels, decrease in abbot curve area and reduction in surface roughness parameters. This study revealed a potential inhibitor of Ets-1 and anti-cancer mechanism of saffron.

Page(s): 1334-1341

DOI: DOI not available

Published: Journal: Journal of Animal and Plant sciences, Volume: 24, Issue: 5, Year: 2014

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