Abstract:
In Pakistan, ticks present a major threat to both livestock and humans, as they can transmit a variety of infections, including those caused by protozoa, viruses, and bacteria such as spirochetes and rickettsia. Although different chemical acaricides have been used to combat ticks, many tick species have developed resistance to these commercially available drugs. This study evaluated the acaricidal effectiveness of methanolic extract from the Trachyspermum ammi plant against ticks at various stages using the adult immersion test (AIT) and the larval packet test (LPT), comparing the results with the positive control, amitraz.The methanolic extract was tested for its acaricidal activity at different timeintervals and concentrations of 5 mg/ml, 10 mg/ml, 15 mg/ml, and 20 mg/ml Both AIT and LPT used the same concentrations. The extract showed a 94%mortality rate in the AIT bioassay and a 99% mortality rate in the LPT bioassay. Distilled water and amitraz served as the negative and positive controls,respectively. At a 24-hour exposure and the highest concentration of 20 mg/ml,the Trachyspermum ammi extract resulted in significant mortality rates of 91%for adult ticks and 90% for larvae, comparable to the 90% mortality caused by amitraz, indicating no significant difference from the positive control. Furthermore, the methanolic extract of Trachyspermum ammi significantly inhibited tick egg-laying ability, with inhibition rates increasing from 20.826%to 85.949% as the extract concentration increased from 5 mg/ml to 20 mg/ml. This study aimed to identify an environmentally friendly acaricide and evaluate the effectiveness of Trachyspermum ammi plant extracts on ticks at different developmental stages. The results suggest that this plant could be a viable alternative to commercially available acaricides.
Page(s):
207-207
DOI:
DOI not available
Published:
Journal: 4th International Conference of Sciences “Revamped Scientific Outlook of 21st Century, 2025” , November 12,2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
Mortality
,
Methanolic extract
,
Ticks
,
Trachyspermum ammi
,
Amitraz
,
Tickborne diseases
,
Oviposition inhibition
,
Acaricidal activity