X-ray inhibits FUT4-mediated proliferation in A549 cells by downregulating SP1 expression | [Journal of Pakistan Medical Association • 2023]

Author(s):

1. Jin-xiao Liang: Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China.

2. Wei Gao: School of Medicine, Zhejiang University City College, China.

3. Wei-tian Wei: Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China.

4. Xun Yang: Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China.

5. Jin-shi Liu: Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China.

Abstract:

Objective: To identify the mechanism of down-regulation of Lewis Y antigen caused by X-ray irradiation. Method: The present original research study was conducted at Zhejiang University City College, Hangzhou, Republic of China, from 2020 to 2022. Western blotting, Co-immunoprecipitation (CO-IP), electrophoretic mobility shift assay and Cell Counting Kit-8 (CCK8) were performed to confirm the effect of X-ray irradiation on A549 cell proliferation and its mechanism. Data was analysed using Statistical Package for Social Sciences (SPSS) 11.5. Results: The expressions of fucosyltransferase IV and Lewis Y were decreased after X-ray irradiation, thus inhibiting the proliferation of A549 lung cancer cells. Deoxyribonucleic acid damage caused by the irradiation caused higher level of poly- adenosinediphosphate-ribosylated Specific Protein 1(SP1), and translocation of SP1 from the nucleus, decreasing the expression of fucosyltransferase IV and Lewis Y. Conclusion: There was a significant role of glycosylation in radiation therapy for lung cancer.

Page(s): 494-499

DOI: 10.3322/caac.21590

Published: Journal: Journal of Pakistan Medical Association, Volume: 73, Issue: 3, Year: 2023

Keywords:

XRay , Lung cancer , SP1 , Fucosyltransferase 4 , Cell proliferation

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