Tracking alien chromosome in sativa background by genomic in situ hybridization. | [Science, Technology and Development • 2004]

Author(s):

1. Fida M. Abbasi: National Agricultural Research Centre (NARC), Islamabad, Pakisan

2. M. Iqbal: National Agricultural Research Centre (NARC), Islamabad, Pakisan

3. M. Salim: National Agricultural Research Centre (NARC), Islamabad, Pakisan

Abstract:

Genomic in situ hybridization (GISH) was used to look into the genomic constitution of monosomic alien - addition line derived from O. saliva x O. brachyantha. Biotin label genomic DNA from O. brachyantha was used as probe. The probe hybridized to the brachyantha chromosome. No detectable bybridizaton signal was observed on sativa chromosomes. This differential painting of chromosome enables us to unequivocally discriminate brachyantha chromosome from those of .saliva. Results showed the usefulness of GISH in the identification of a single alien chromosome in the sativa background.

Page(s): 64-66

DOI: DOI not available

Published: Journal: Science, Technology and Development, Volume: 23, Issue: 2, Year: 2004

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