Abstract:
Inflammation is a natural host immune response to pathogens attack, cell injury and entry of noxious substances in to the body. To cure inflammation various drugs have been introduced in the market by pharmacists, but they have side effects. However, some of the medicinal plants contain numerous phytochemicals, including polysaccharides that have therapeutic effects. Salvia hispanica L., seeds that are commonly known as chia seeds release mucilage after in contact with water. The mucilage generally consists of 85-90% of carbohydrates and some are reported as potent bioactive compounds. This study was, therefore, designed to extract and fractionate the bioactive polysaccharides from the chia seed mucilage and to establish their in vivo and in vitro anti-inflammatory role. The mucilage was collected after soaking the seeds in distilled water and precipitated with 75% ethanol. The ethanol precipitated fraction was resuspended in water and fractionated through Bio Gel P10 (90µm-180µm, pore size).A total of 60 fractions were obtained, which contained saturated and unsaturated uronic acid, pentoses and hexoses. The fractions were tested for In vitro anti-inflammatory potential through HRBCs membrane stabilization assay, while the in vivo activity was performed using carrageenan induced paw edema model in mice. The results showed that the fraction Ch19 significantly (P=0.05) stabilized the HRBCs membrane (EC50=8.75 µg/mL). Similarly, application of fraction Ch39 at a concentration of 1000 µg/mL resisted (97.20%) the heat induced destabilization of HRBCs membrane. The results of in vivo anti-inflammatory activity revealed that fractionCh31 significantly reduced the volume of the mice paw edema by 91.37%. The results indicate that the ethanol precipitated fractions of chia seed mucilage have medium sized polysaccharides with anti-inflammatory potential. These medium sized polysaccharides from chia seed mucilage appear to be promising candidates for further preclinical and clinical trials to treat inflammation and inflammation mediated diseases.
Page(s):
4-4
DOI:
DOI not available
Published:
Journal: International Food & Nutrition Conference" August 18th-20th, 2022, University of Swat, Swat (Book of Abstracts), Volume: 1, Issue: 1, Year: 2022
Keywords:
mucilage
,
inflammation
,
Chia
,
Polysaccharides