Abstract:
Medicinal plants being globally reliable drug candidates, with low toxicities and high efficacies, can be explored for their microbicidal properties to cater growing bacterial resistance against available antibiotics. South Asia is considered as the main region of prevailing microbial resistance towards antibiotics. Antimicrobial potential has been attributed to plants due to the presence of secondary metabolites such as alkaloids, flavonoids, tannins and polyacetylenes. Bistorta amplexicaulis being an herbal drug has been explored for antioxidant, antitumor, anticancer, antityrosinase activities and is used to treat muscle injuries, rheumatism, osteoporosis, fever and measles in folkloric medicine. Current investigation was performed to carry out the polarity wise extraction of rhizome powder of Bistorta amplexicaulis with different solvents (n hexane, ethanol, acetone, aqueous and ethyl acetate). Moreover, the extract was evaluated using LC-MS for probable phytochemicals present in it. Antibiotic susceptibility of the enteric bacterial pathogens was assessed using disc diffusion method. Antimicrobial activity was evaluated by well diffusion method. The results indicated that the aqueous extract showed highest %yield (54.9%) and the extract of n-hexane showed lowest %yield (0.87%) among other extracts. In this study, susceptibility test was performed using antibiotic discs ampicillin (10µg), tylosin (30µg), oxytetracycline (30µg), gentamicin (10µg), cefoxitin (30µg), fluconazole (25µg). All strains showed resistance against ampicillin and fluconazole. Whereas some strains showed resistance against tylosin except Klebsilla pneumoniae (AC1). Then, the antibacterial activity of extracts was evaluated against five enteric pathogens like Klebsiella pneumoniae (AC1), Klebsiella quasipneumoniae(AC2), Klebsiella pneumoniae (AC3), Klebsiella pneumoniae (AC4) and Enterobacter cloacae (AC5’). Current study doesn’t report antibacterial activity of n-hexane and aqueous extracts against these five strains. While the highest antibacterial activity was observed against Enterobacter cloacae (AC5’) with ethanol extract (8µg/mL) 23mm±1.73. In conclusion, phytochemicals present in Bistorta amplexicaulis offer a solution to antimicrobial resistance but their mechanism of action should needs further analysis.
Page(s):
68-68
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
Antibacterial activity
,
Antibiotic resistance
,
susceptibility test
,
Bistorta amplexicaulis
,
Enteric Pathogens