Abstract:
The present study was conducted to compare the anti-fungal and anti-bacterial activity of environmentally friendly biosynthetic silver nanoparticles from leaves extracts of Azadirachta indica and Mentha arvensis. Fine pieces of leaves of neem and mint were used to prepare aquatic decoction in 1L distilled water in separate flask. In each flask, 1mM AgNO3 solution was mixed in each of the aqueous leaf extract of A. Indica and M. arvensis and stirred continuously at pH 7.0 at 25°C to 40°C until yellowish green color change into brown. Brown color is a strong indication of AgNPs biosynthesis. The Characteristics of AgNPs were studied, by five different techniques including UV-Vis spectrophotometer, an EDX, an XRD, FTIR, and a scanning electron microscope (SEM). The anti-fungal and anti-bacterial activities were determined by disc diffusion method. UV-Vis spectrophotometer confirm the synthesis AgNPs whereas EDX analysis further confirm the purity of AgNPs. FTIR analysis provided the functional group of phytochemicals of plant extracts involved in reducing and stabilizing the AgNPs. By XRD analysis. The results of XRD analysis showed the crystalline nature of AgNPs, the size of nanoparticles was also estimated which was at the average of 24.21nm and 21.64nm of Mentha arvensis and Azadirachta indica, respectively, whereas the scanning electron microscopy (SEM) images represented the irregular shape and rectangular segments fused together from Mentha arvensis and Azadirachta indica, respectively. The comparison proved that Azadirachta indica mediated AgNPs have higher potential for anti-bacterial and anti-fungal activities than Mentha arvensis mediated AgNPs.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
antibacterial
,
antifungal activities
,
neem
,
Nanoparticles
,
leaf extract
,
Mint