Abstract:
Gut microbiome is the community of microorganisms present inside our gut, roughly 1100 gut microbial species have a critical role in a wide range of physiological processes. Through neuronal, endocrine, and inflammatory mechanisms, the gastrointestinal tract and the central nervous system can talk to one another. The brain-gut-microbiome axis was first proposed as a result of preclinical studies that demonstrated evidence of bidirectional communication between the brain and gut bacteria. Disturbances in the brain, stomach, and microbiota have been linked to depression. This research’s goal is to assess how healthy, unstressed mice`s feces affect stressed mice. FMT is used to replenish the gut with healthy bacteria in order to enhance the condition of the microbiome. Firstly, experimental mouse was divide into two groups that is control and stress group. Mouse of stress group were subjected to chronic stress for 4 weeks by reversing their normal circadian rhythm by giving light and dark stressor before a behavioral investigation was performed. While the mice were stressed, fecal suspension of healthy mouse were orally administered for seven consecutive days. The behavior study was then repeated. To examine the impact of stress on the tissues of the gut, the histological investigation of the intestinal tissue samples was also carried out. DNA was extracted from the stool sample of each group of mouse that is stressed, control, and treatment group. Following this, the DNA was amplified using 16s rRNA, Bifidobacterium and Enterobacteriaceace primers. The amplified DNA bands were then analyzed using Image J software.The findings showed that FMT treatment relieved the mice`s depressed symptoms.The findings showed that FMT treatment relieved the mice`s depressed symptoms.
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Journal: Abstract Book on 9th Annual Neuroscience Conference (ANC-23) August 12-13, 2023 , Volume: 0, Issue: 0, Year: 2023