Author(s):
1. Semiha Dede:
Department of Biochemistry, Faculty of Veterinary Medicine, Van Yuzuncu Yil University,Van,Turkey
2. Vedat Turkoglu:
Department of Chemistry, Faculty of Science, Van Yuzuncu Yil University,Van,Turkey
3. Zehra Bas:
Department of Nutrition and Dietetics, Faculty of Health Sciences, Van Yuzuncu Yil University,Van,Turkey
Abstract:
Angiotensin converting enzyme (ACE, EC 3.4.15.1) is an important enzyme responsible for regulating blood pressure. Inhibition of this enzyme is an important treatment approach in the treatment of hypertension, and natural or synthetic ACE inhibitors are often used for this purpose. In this study, the preventive effect of two important antioxidant compounds, lycopene (LYC) and thymoquinone (TQ) on ACE activity in human plasma was investigated. Human plasma was used as ACE source. ACE activity was calculated absorbance at 345 nm after incubation for 30 minutes at 35°C. TQ and LYC showed inhibitory effect on ACE activity. IC50 values for TQ and LYC were determined as 314µM and 182 µM, respectively. Type of inhibition for TQ and lycopene from plot Line weaver-Burk was designated as noncompetitive inhibition. The Ki constants of TQ and LYC were determined to be 707 µM and 167 µM, respectively. It was concluded that TQ and LYC may have significant potential as ACE inhibitors.
Page(s):
801-805
DOI:
DOI not available
Published:
Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 35, Issue: 3, Year: 2022
Keywords:
Inhibition
,
In vitro
,
Thymoquinone
,
lycopene
,
Angiotensin converting enzyme
References:
References are not available for this document.
Citations
Citations are not available for this document.