Xiao-Gao-Jiang-Zhuo-containing serum inhibits adipogenesis through SIRT1-IGF-1 crosstalk in 3T3-L1 preadipocytes | [Pakistan Journal of Pharmaceutical Sciences • 2023]

Author(s):

1. Liang Chen: Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences,Beijing,China

2. Yu Han: Hospital Administration Office, China Academy of Chinese Medical Sciences,Beijing,China

3. Jing Li: Department of Endocrinology, Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University,Urumqi,China

4. Chunpeng Feng: Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences,Beijing,China

5. Chen Chen: Department of Endocrinology, Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University,Urumqi,China

6. Ting Ye: Department of Endocrinology, Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University,Urumqi,China

Abstract:

PPAR?, CEBP/a, and SREBP1C are the major transcriptional factors participating in adipogenesis and lipogenesis. SIRT1 and IGF-1 signaling pathways are important pathways involved in body endocrine and metabolism. Our unique Chinese herbal medicine Xiao-Gao-Jiang-Zhuo (XGJZ) has a remarkable clinical effect on obesity. However, the molecular basis remains unknown. XGJZ-containing serum was treated in the incubation of 3T3-L1 preadipocytes to observe its function in the 3T3-L1 cell differentiation. Oil Red O staining was used to monitor the lipid droplets accumulated after 8 days of incubation. RT-qPCR and western blotting were used to investigate the regulatory effects of XGJZ-containing serum on adipogenesis-related factors. The protein levels of main molecules in SIRT1 and IGF-1 signaling pathways were also detected by western blotting. XGJZ-containing serum notably suppressed the lipid accumulation in differentiated adipocytes through SIRT1/IGF-1 pathway. XGJZ-containing serum activated the SIRT1/IGF-1 pathway and reduced the expression levels of PPAR?, CEBP/a, and SREBP1C through this pathway. Additionally, XGJZ-containing serum enhanced the phosphorylation of ATGL and HSL and then induced lipolysis. XGJZ-containing serum has inhibitory effects on adipogenesis in 3T3-L1 preadipocytes through SIRT1/IGF-1 signaling pathway. Our study affirmed the effect of XGJZ-containing serum in the treatment of obesity. It provides a basis for the mechanism of obesity.

Page(s): 773-782

DOI: 10.36721/PJPS.2023.36.3.REG.773-782.1

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 36, Issue: 3, Year: 2023

Keywords:

Obesity , Lipid metabolism , XGJZ , adipogenesis , SIRT1 , IGF1

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