Thyroid stimulating hormone stability in serum | [Journal of College of Physicians and Surgeons--Pakistan : JCPSP • 2001]

Author(s):

1. Farooq Ahmad Khan: Department of Pathology and Blood Transfusion Service, PNS Shifa Hospital, Karachi, Pakistan

2. Asma Shaukat: Department of Pathology, Quaid-i-Azam Medical College, Bahawalpur, Pakistan

3. Sameena Ghayur: Combined Military Hospital,Okara,Pakistan

Abstract:

Thyroid Stimulating Hormone (TSH) is a thermolabile peptide hormone. It is unstable in serum and rapidly degrades when exposed to ambient temperature (temp) for considerable time. The stability of TSH with regard to storage temp, duration and added preservative was evaluated for performing TSH assay. Venous blood was collected in 5 ml plain and aprotinin containing glass tubes from 37 individuals aged 15-56 years. Serum obtained was analysed for TSH at zero time value, then divided into 3 aliquots, sets with and without aprotinin. One set was kept at room temperature (RT), 2nd at 4°C and 3rd at -20°C. TSH was measured after 24 and 72 hours for comparison to the zero time value of TSH. Significant decline in TSH was seen in the samples stored at RT for 72 hours. This effect was abolished when aprotinin, the protease inhibitor, was added to the samples. No significant difference from zero time value was noticed in the aprotinin-treated or untreated sera when kept at RT for 24 hours or when stored at 4°C or -20°C for 72 hours. Thus we concluded that proper storage and addition of aprotinin may significantly reduce TSH degradation.

Page(s): 20-22

DOI: DOI not available

Published: Journal: Journal of College of Physicians and Surgeons--Pakistan : JCPSP, Volume: 11, Issue: 1, Year: 2001

Keywords:

laboratory , Hormones Thyrotrophin Thyroidstimulating hormones Stabilizing agents Aprotinin Diagnosis

References:

[1] Young DS,Bermes EW,Ashwood ER .1994 .Specimens collectionand processing; Sources of biologicalvariation. Saunders Co, 2 : 58-101.

[2] Khan AB,Iqbal J . .. HealthyPopulation; RefvaluesofThyroid Hormones. The Professional1999;, 4 : 536-41.

[3] Khan DA,Dilawar M,Khan FA,Livsey JH,Hodgkinson SC .1980 .prolactinand insulin in plasma. Ann Ciin Biochem, 4(1) : 151-5.

[4] Jackson MD .1982 .Thyrotropin-releasinhgormone. NEngJMed, 306 : 145-55.

Citations

Citations are not available for this document.

Citations

Downloads

Views