Abstract:
Hepatitis 13 virus is one of the main etiologic factors involved in the development of human hepatocellular carcinoma. FIBV has eight different genotypes that are geographically distributed. Hepatitis B surface antigen is composed of three related envelope proteins that are synthesized by alternate use of three translational start codons and a common stop codon. PreS region at the 5` end of envelope gene is highly immunogenic with live known epitopes and hepatocellular binding sites., For cloning and expression studies of PreS gene, primers were designed that contained restriction sites and a termination codon at 3` end. Pakistanian 1113V PreS gene was PCR amplified and cloned in a T-A cloning vector. Clones were screened and positive clones were double digested with 1-lindlll and LcoRl. Gel purified insert was cloned in high expression vector pKK223-3. Cloning was confirmed with restriction analysis and PCR amplification. Such clones would be useful for expression study of PreS product in bacterial cell, for large-scale production of viral antigen I`or diagnostic purposes and possibly vaccine production.
Page(s):
165-168
DOI:
DOI not available
Published:
Journal: Pakistan Journal of Zoology, Volume: 37, Issue: 3, Year: 2005