-Depleting energy resources worldwide has created a huge concern towards finding alternative, innovative solutions. Microbial fuel cells (MFCs) has been viewed as another innovative solution to this ongoing dilemma. Enrichment of electricigens and their associated power generation was carried out in Microbial Fuel Cells (MFC1 and MFC2) from sewer contaminated soil sediments (S1 and S2). Microtiter plate assay indicated 90% (n=101) biofilm forming culturable bacteria and most common of them were Pseudomonas, Bacillus sp., Proteus sp., and E. coli. In MFC1, Pmax = 26728mWm-2 whereas in MFC2 Pmax = 12008mWm-2(57?). Molecular phylogeny of biofilms in MFC1 and MFC2 indicated abundance of phylum Proteobacteria. Its density in MFC1 was comparatively higher [(99.1%) (Class: ß-Proteobacteria > ?-Proteobacteria > a-Proteobacteria) than in MFC2 [(94.5%) (Class: ?Proteobacteria > ß-Proteobacteria > Opitutae)]. Presence of few exotic species like proteobacterium_cloneBAS3181, uncultured a-proteobacterium cloneMS129A1-D01 and uncultured pseudomonas sp._clone _G13-S-2-F05 with unknown functionality specifically demanded physiological role in MFCs. The present study revealed the successful enrichment and power generation capabilities of electrogenic bacterial communities enriched from sewer contaminated soil sediments (S1, S2). Bacterial density, diversity and associated power output varied with different soil sediments.