Abstract:
Antibiotic resistance by microorganisms is a global issue, and the emergence of antibiotic-resistant bacterial strains poses an alarming threat to the healthcare system. Proper strategies are needed to manage multi-drug resistant (MDR) bacterial infections. Nanotechnology is considered an advanced approach due to the smaller size of nanoparticles, which can play a crucial role as drug carriers, delivering antibiotics to the target site of resistant organisms and reducing the phenomenon of antibiotic resistance. The current research study focused on the biosynthesis of TiO2 nanoparticles (NPs) using Syzygium aromaticum extract and determining their capability to enhance the activity of commercially available antibiotics (meropenem, amikacin, ampicillin, amoxicillin-clavulanic acid) against MDR Salmonella typhi. A green synthesis approach was used for the synthesis of TiO2 NPs using Syzygium aromaticum extract. Furthermore, standard techniques were used for the characterization of synthesized TiO2NPs in the Centralized Resource Laboratory, Peshawar. Additionally, the antimicrobial activity of coated and non-coated antibiotic discs against MDR S. typhi was evaluated using the standard agar well diffusion method. In the current research study, UVVisible spectroscopy revealed an absorption peak at 310 nm within the prescribed range, confirming the synthesis of TiO2NPs. Scanning electron microscopy (SEM) demonstrated the morphology of TiO2NPs, while energy dispersive analysis of X-rays (EDAX) quantified the presence of titanium, carbon, and oxygen in the synthesis of TiO2NPs. Fourier transform infrared spectroscopy (FTIR) confirmed the capping and reduction of TiO2NPs through the presence of phenolic, carboxyl, and hydroxyl groups. Moreover, it was observed that TiO2NPs significantly enhanced the antibacterial activities of the test antibiotics against MDR S. typhi (p = 0.01). This was confirmed by comparing the zones produced by non-coated antibiotics with those produced by TiO2-NPs coated antibiotics. It was concluded that TiO2NPs synthesized from S. aromaticum extract could be of great importance in pharmaceutical and medical applications due to their biocatalytic activity.
Page(s):
319-319
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
antibiotics
,
SEM
,
FTIR
,
MDR S typhi
,
S aromaticum extract
,
Bioassay
,
TiO2 NPs