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Standrization of phytase and biomass production in submerged fermentation with aspergillus flavus Phy190 isolated from soil
Author(s)
Zahid Manzoor Department of Parasitology and Microbiology,,
Aftab Ahmad Anjum Institute of Microbiology, University of Veterinary & Animal Sciences, Lahore, Pakistan
Syeda Maryam Hussain Department of Livestock Production and Management
Muhammad Zishan Ahmad Department of Livestock Production and Management
Murtaz Ul Hassan Department of Parasitology and Microbiology
Muhammad Kamran Department of Parasitology and Microbiology
Muhammad Shoaib Department of Parasitology and Microbiology
Aayesha Riaz Department of Veterinary Pathology
Zaib Ur Rehman Department of Poultry Science
Ayesha Qaisar Department of Zoology, Wildlife and Fisheries, PMASAAU, Rawalpindi, Pakistan
Ali Ahmad Microbiology Section, Department of Pathobiology, Narowal Campus, University of Veterinary & Animal Sciences, Lahore, Pakistan
Abstract
Phytase is a type of enzyme categorized under the phosphatase group that facilitates the breakdown of phytic acid, leading to the liberation of inorganic phosphorus that can be utilized by the host. In this study we standardized the production of phytase and biomass (estimated by the dry weight content g/L) by submerged fermentation with Aspergillus flavus PHY190 which was isolated from soil. Experimental assays were performed under diverse conditions of pH (3.0 to 6.0) and temperature (20 to 45oC), and the influence of these fluctuating conditions on the responses was studied through one variable approach. Outcome of temperature, pH, substrate type and concentration were observed using submerged fermentation. The maximum production of phytase was achieved when using pH 5.0, temperature 300 C and 4% oat bran as substrate. Phytase activity was increased in up to 3.68- fold and the biomass matter was increased in up to 3.78-fold. Bye-products of Agricultural industry such as oat bran can be used alternative for cost efficient phytase production method under optimized conditions. One-way Analysis of Variance (ANOVA) and Post-Hoc Multiple comparison test (Duncan) was performed to compare the differences between means using SPSS. Therefore, these results are of interest as they contribute for the development of an industrial process for phytase production with eminent yields by submerged fermentation.
Publication Details
Page(s) 118-118
DOI DOI not available
Published Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords
Aspergillus flavus Oat bran phytase Submerged fermentation
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