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The influence of intravenous lipopolysaccharide injection on TLR4 transcription levels in duck (Anas domestica) liver, spleen and bursa of Fabricius.
Author(s):
1. Jun-Ying Liu: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China
2. He-He Liu: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China
3. Jie Kou: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China
4. Ji-Wen Wang: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China
5. Liang Li: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China
6. Xiao-Hui Du: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China
7. Yang-Mei Zhao: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China
Abstract:
TLR4 is a member of the toll-like receptor family and can recognize lipopolysaccharide (LPS), the main component of gram-negative bacteria. To investigate how TLR4 responds to LPS, the TLR4 transcription levels in duck liver, spleen and bursa of Fabricius were examined after a model for immune stress was implemented through LPS injection in Peking ducks. The results from this assay suggested that LPS injection significantly increased the concentration of tumor necrosis factor-a (TNF-a) in bursa of Fabricius (P0.05). Furthermore, correlation analysis showed a positive relationship between the mRNA expression of TLR4 and concentrations of TNF-a and IL-12. These results revealed that although there were no significant effects of LPS injection on TLR4 mRNA expression in lymphoid organs, the response to LPS may not mainly rely on the transcription of TLR4, and the downstream molecular response in the immune system may contribute more to the TLR4 signaling cascade.
Page(s): 1237-1244
Published: Journal: Pakistan Journal of Zoology, Volume: 50, Issue: 4, Year: 2018
Keywords:
Peking duck , lps , Interleukin12 , Immunity index , Tolllike receptor 4 , Tumor necrosis factor a , ImmunoglobulinG
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