Author(s):
1. Farah Ashraf:
Lahore University of Management Sciences, Lahore, Pakistan.
2. Ismat Nawaz:
Lahore University of Management Sciences, Lahore, Pakistan.
3. Shaper Mirza:
Lahore University of Management Sciences, Lahore, Pakistan.
4. G. A. Chotana:
Lahore University of Management Sciences, Lahore, Pakistan.
5. Tariq Mehmood:
Shalamar Hospital Lahore, Pakistan
Abstract:
Uropathogenic Escherichia coli (UPEC) causes urinary tract infections (UTIs) in approximately 150 millionpeople worldwide each year. It is estimated that 40% of women will experience at least one UTI during their lifetime. UTIs are caused by a wide range of pathogens, including Gramnegative and Gram-positive bacteria presenting a significant public health challenge. E. coli is the predominant causative agent of both uncomplicated and complicated UTIs. In an era of increasing bacterial resistance to antimicrobial agents, coupled with a high prevalence of multidrug-resistant (MDR) strains in community and hospital-acquired infections, it is essential to re-evaluate existing antimicrobial agents. In this context, the use of Sulfamethoxazole derivatives appears to be a reasonable approach. This study aimed to evaluate the activity of newly synthesized fluorinated sulfonamides against common uropathogens, comparing them to the widely used Sulfamethoxazole and other antimicrobial agents routinely used for UTI treatment. The bactericidal activity of our sulfonamide compounds against resistant UPEC strains was assessed by determining the Minimum Inhibitory Concentration (MIC) and comparing their effectiveness to standard antibiotics. We hypothesized that the synthetically prepared fluorinated sulfonamides would exhibit significant bactericidal activity against resistant UPEC strains. Among the compounds tested, Compound 4g demonstrated lower MIC values compared to Sulfamethoxazole when tested against Gram-negative bacteria, including clinical MDR E. coli strains. The MIC values for Compound 4g ranged from 62.5-125 µg/mL for Uropathogenic E. coli strains, while the MIC for Sulfamethoxazole exceeded 500 µg/mL for the same resistant strains. To identify the antibacterial component of the active compound, we performed activity assays with and without the presence of nitro groups. Our results showed that the nitro group was crucial for the compound's activity, as the removal of the nitro group from Compound 4g led to a complete loss of antibacterial activity. Therefore, our study confirmed that the antibacterial properties of Compound 4g are attributed to the nitro group, rather than the sulfonamide moiety.
Page(s):
81-81
DOI:
DOI not available
Published:
Journal: 4th International Conference of Sciences “Revamped Scientific Outlook of 21st Century, 2025” , November 12,2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
coli
,
sulfonamides
,
Uropathogenic E
,
Minimum inhibitory concentration MIC
,
Nitro groups
,
Multi drug resistant Bacteria
References:
References are not available for this document.
Citations
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