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Alphamethrin Toxicity in Tribolium castaneum Larvae.
Author(s):
1. Naveeda Tufail: Zoology Department, University of Punjab, Lahore, Pakistan
2. A. R. Shakoori: Zoology Department, University of Punjab, Lahore, Pakistan
Abstract:
Effectof sublethal doses (1000 and 2000 ppm) of alphamethrin (Bestox 5EC) were studied on 6th instar larvae of insecticide- susceptible (FSS II) and an organophosphorous insecticide-resistant strain (PAK strain) of Tribolium casstneum. In FSSII strain larvae the lactase, acid phosphatase (AcP) and alanine aminotransferase (ALAT) activities were increased, whereas aspartate aminotransferase (ASAT) and cholinesterase (ChE) activities were significantly decreased at low doses (1000 ppm). At higher sublethal dose (2000 ppm) alkaline phosphatase (AkP) was drastically decreased and protease increased. Both the doses of alphamethrin decreased trehalose, glycogen, lipids, cholesterol, proteins and free amino acids (FAA) contents, while increased the RNA content. In PAK strain larvae, however, almost all carbohydrases (viz., amylase, invertase, lactase and maltase). AcP, ASAT, lactate dehydrogenase, isocitrate dehydrogenase and carboxylesterase showed significant increase at all concentrations of insecticide, while trehalase, ChE and protease activities were raised at 2000 ppm. The two sublethal doses of alphamethrin decreased glucose and glycogen contents, while increased cholesterol, soluble protein and DNA contents. A significant increase in trehalose and lipid contents. and significant decrease in total protein and FAA contents was also recorded at 2000 ppm.
Page(s): 59-70
DOI: DOI not available
Published: Journal: Pakistan Journal of Zoology, Volume: 24, Issue: 1, Year: 1992
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