Toxic effect of caffeine on the cardiac muscle tissue of albino rats | [International Journal of Biology and Biotechnology • 2024]

Author(s):

1. Aly Khan: CDRI, Pakistan Agricultural Research Council, University of Karachi, Karachi-75270, Pakistan

2. Nabeel Mohammad Ghanem: Department of Medical Laboratories, Faculty of Applied Sciences, Turba Br. Taiz University, Taiz Yemen

3. Shagufta Ambreen Sheikh: Microbiology Section, PCSIR Laboratories, Karachi, Pakistan

4. Samina Waheed: Department of Zoology, University of Karachi, Karachi-75270, Pakistan

5. Tanveer Abbas: Department of Microbiology, University of Karachi, Karachi-75270, Pakistan

Abstract:

Use of caffeine and other energy increasing compounds have become very popular amongst younger generation over the last few decades. In United States of America caffeine is considered to be one of the most important stimulants which may result in metabolic disorders, cardiovascular diseases, Parkinson disease etc. Caffeine three treatments were tested to observe histological changes, 3 mg/0.5 mL; 9 mg/3 mL and 125 mg/3 mL of distilled water on the cardiac muscles of albino rats. Untreated albino rats were kept as control. The rats were anesthetized with chloroform 3 mL. In dead and anesthetized rats an incision was made in the abdomen and incised up to neck. Gross sections of heart muscular tissues was observed, and the tissues were taken and cut in longitudinal and transverse pieces and selected for tissue processing, block making, sectioning, mounting, staining, microscopic observation and photography. The healthy cardiac muscles of albino rats showed normal and centrally arranged nucleus, the cardiac muscle fibres were arranged properly and connective tissue appeared normal. Several histopathological alterations were observed in cardiac tissue of treated albino rats. The cardiac muscle tissue of albino rats treated with 3 mg/0.5 mL of caffeine showed formation of spaces due to shrinkage of muscle fibres. The nucleus became more obvious due to the shrinkage of muscle fibres. In rats treated with 9 mg/3 mL of caffeine showed the hyaline degeneration of the arterial wall. At the same time there were spaces seen in between the muscles indicating atrophy. In rats treated with 125 mg/3 mL of caffeine heart tissue showed atrophy and condensation of tissue muscles fibres surrounding vascular area. The arterial wall showed hyaline degeneration. Damaged heart tissue observed in the present study was due to different doses of caffeine administrated to the albino rats.

Page(s): 59-64

DOI: DOI not available

Published: Journal: International Journal of Biology and Biotechnology, Volume: 21, Issue: 1, Year: 2024

Keywords:

Albino rats , Caffeine , Caffeine , Histopathology , cardiac muscle tissue

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