Abstract:
Brassica vegetables and oilseed crops from family Brassicaceae, a large family comprising of 350 genera of economically important species includes important and nutritional vegetable crops grown worldwide and Pakistan. They are not only used for oil production or vegetables for humans but are also used as forage or fodder for animals, birds or even poultry. They are the third most important edible oil source covering 15% of global oil needs. The important brassica species from triangle of U includes 3 diploid species as B. rapa(AA genome, 2n = 18), B. nigra (BB genome, 2n = 16)and B, oleracea (CC genome, 2n = 18), and 3 allotetraploid species as B. juncea (AABB genome, 2n = 36), B. napus (AACC genome, 2n = 38)and B. carinata (BBCC genome, 2n = 34). The important brassica vegetables used across globe are mustard, turnip, cauliflower, cabbage, broccoli, collards, kale and kohlrabi. The genomic composition and diversity of Mobile Genetic Elements (MGEs) in these crops is not fully known, which have contributed totheir genome size duplication, evolution, diversification, and plasticity. The current work focused on the identification of diversified MGEs in various plants by exploiting the bioinformatics, molecular and cytogenetic techniques. Of theLTR retrotransposons, the superfamilies like Copia, Gypsy, and retroviruses (caulimoviruses and pararetroviruses) were abundantly proliferating in almost all Brassica genomes. AmongNon-LTR retrotransposons, superfamilies SINES and LINES predominated, while DNA transposons were abundantly represented by CACTA, hAT, Harbinger, Mariner, Mutator and MITEs super families, while Helitron, Politron and Maverik were rarely identified. PCR amplification of reverse transcriptase (RT) of Retrotransposons and transposase (TNP) of DNA transposons revealed their distribution among various genomes, with some elements were found to be specie or genera specific, while others were mobilized by horizontal transfer. The evolutionary relationship of these elements resolved them into superfamily and family specific lineages. The results enabled the characterization, annotation, evolutionary dynamics, and structural features of MGEs and their derivatives in Brassica crops.
Page(s):
326-326
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
genome
,
Gypsy
,
bioinformatics
,
Evolution
,
Retrotransposons
,
Retroviruses