Abstract:
Medicinal plants have gained prime importance due to the presence of secondary metabolites which are in use to cure different ailments. Trichodesma indicum (L) is an annual herb that is famous due to its medicinal importance because plant contained potent phytoconstituents which are responsible to improve human health. The current study was designed to evaluate the biological potential of bioactive compounds in the selected fractions which were tested by performing different biological activities like antioxidant, anti-inflammatory, anti-proliferative, hepatotoxicity and compound composition were quantized by using non- destructive techniques like FT-IR, HPLC and GC-MS. The results showed that the chloroform and ethyl acetate fractions of T. indicum were rich in TPCs (total phenolic content) i.e. 499.80 and 744.65 mg of gallic acid equivalents per g, respectively, while TFCs (total flavonoid content) were 42.48, and 55.02 mg of catechin equivalents per g, respectively. HPLC analysis confirmed the presence of phenolics i.e. chlorogenic acid, ferulic acid, caffeic acid, cinnamic acid, gallic acid and flavonoids i.e. myricetin, Quercetin in selected fraction of T. indicum. FTIR functional group analysis further confirmed authenticity of HPLC results. Significant antioxidant potential was analyzed by performing ABTS, DPPH, and H2O2 assays. The highest % inhibition of inflammation was observed in ethyl acetate fraction by performing in-vitro and in-vivo assay, 87.57 % and 79.18 % (P < 0.05) respectively. Thrombolytic activity of the T. indicum fractions were observed in range from 9.85 % to 27.26 %. Similarly, hemolytic activity ranges from 0.70 % to 13.07 %. The % cell viability of MCF-7 (Breast cancer cell line) was observed by MTT assay of ethyl acetate fraction (50.96 %) and chloroform fraction (58.62 %) compared to control (99.9%). LD50 value was estimated to check the toxic effect of plant fractions. GCMS coupled with molecular docking depicted the inhibition of COX-1 and COX-2 by the GC-MS-identified compound 9,19-Cyclolanost-24-en-3-ol, (3. Beta.)- (-9.6) that proved the significant effect to prevent inflammation based on the computed binding energies of the compounds. The in-silico anti-proliferative analysis was conducted by using BRCA1 and BRCA2 cancerous protein against GC-MS identified compounds among which stigmasterol (-8) was observed for high binding affinity.The above said study showed that phytochemicals in T. Indicum are latent source of pharmaceutically important compounds with potential for multiple pharmacological applications.
Page(s):
0-117
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
HPLC
,
GCMS
,
antiinflammatory activity
,
Antiproliferative activity
,
Trichodesma indicum
,
docking techniques