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Quantification of Aflatoxins in Different Dates (Phoenix Dactylifera L.) Varieties from Humid Subtropical Regions.
Author(s):
1. Shamayem Aslam: Institute of Molecular Biology & Biotechnology, The University of Lahore,Lahore 54000,Pakistan
2. Muhammad Yasin Ashraf: Institute of Molecular Biology & Biotechnology, The University of Lahore,Lahore 54000,Pakistan
3. Muhammad Rafique Asi: Food Toxicology Lab, Nuclear Institute for Agriculture and Biology,Faisalabad 38000,Pakistan
4. Shabbir Hussain: Central Analytical Facility Division, Pakistan Institute of Nuclear Science and Technology (PINSTECH),P.O. Box Nilore, 1482, Islamabad,PakistanFood Toxicology Lab, Nuclear Institute for Agriculture and Biology,Faisalabad 38000,Pakistan
5. Hafiz M. Fahad Raza: Food Toxicology Lab, Nuclear Institute for Agriculture and Biology,Faisalabad 38000,Pakistan
6. Muhammad Adil Rehman: Department of Food Science and Technology, University College of Agriculture & Environmental Sciences, The Islamia University of Bahawalpur,Bahawalpur 63100,Pakistan
7. Sumera Anwar: Institute of Molecular Biology & Biotechnology, The University of Lahore,Lahore 54000,Pakistan
8. Fahad Shafiq: Institute of Molecular Biology & Biotechnology, The University of Lahore,Lahore 54000,Pakistan
Abstract:
Food items are prone to fungal attacks due to the warm and humid climatic conditions of Pakistan. The aflatoxins (AFs) are the most common mycotoxins produced by fungal species highly responsible for liver toxicity and hepatocellular carcinoma. The present study was planned to assess the contamination levels of aflatoxins (AFB1, AFB2, AFG1, and AFG2) in local and imported varieties (Irani and Saudi) collected from Pakistan. About 251 dates samples were procured from the markets and analyzed by reverse-phase high performance liquid chromatography coupled with a fluorescence detector (HPLC-FLD) in isocratic mode. The AFs were detected in 89% of date samples ranging from 32.9 to 1465.4 µg kg-1 all of which exceeded the permissible limit of AFs set by FDA (USA) (20 µg kg1) and EU (4 µg kg-1) for human consumption. The two main local varieties Kupro (801.5 µg kg-1), and Mazafati (1275.8 µg kg-1) had maximum concentrations of total aflatoxins. Out of imported varieties, Mabroom (Saudi) (272.94 µg kg-1) and Rubai (Irani) (1465.42 µg kg-1) have the highest concentrations of total aflatoxins. The maximum levels of AFB1 were seen in Mazafati (Pakistani) (521.5 µg kg-1), Mabroom Saudi (127.6 µg kg-1) and Rubai Irani (662.8 µg kg-1). It could be seen that overall local varieties have higher contamination of AB1 and total AFs with the exception of Rubai (Irani) and Mabroom (Saudi) due to the poor storage conditions, improper pre- and post-harvesting handling, and humid conditions of Pakistan emphasizing the need for proper management and regulation. This study could be taken towards the analysis of risk assessment of Pakistani population on greater scale and also to determine more fungal susceptible Pakistani date varieties.
Page(s): 39-39
DOI: DOI not available
Published: Journal: International Food & Nutrition Conference" August 18th-20th, 2022, University of Swat, Swat (Book of Abstracts), Volume: 1, Issue: 1, Year: 2022
Keywords:
Aflatoxins , Aflatoxins , Dates Phoenix Dactylifera L , Humid Subtropical Regions
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