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A new RP-HPLC method for analysis of meloxicam in tablets.
Author(s)
M. Saeed Arayne Department of Chemistry, University of Karachi, Karachi, Pakistan
Farhan Ahmed Siddiqui Department of Chemistry, University of Karachi, Karachi, Pakistan
Najma Sultana Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan
Abstract
A simple and rapid high-performance liquid chromatographic (HPLC) method for the determination and quantification of meloxicam has been developed. The chromatographic system consisted of a Shimadzu LC-l0 AT VP pump, SPD-10 AV VP UV/ visible detector, and a CBM-I02 Bus Module integrator. Separation was achieved on the Il Bondapak 125 A C18 10 µm column at room temperature. The sample was introduced through an injector valve with a 10 µl sample loop. Methanol:water (70:30 v/v) was used as mobile phase, with flow rate 2 ml/minutes pH was adjusted to 2.6 with phosphoric acid. U.V. detection was performed at 230 nm. The results obtained showed a good agreement with the declared content. Recovery values of meloxicam in tablets (in Melfax 15 mg tablets) were from 99.27 % to 100.06 %. Piroxicam was used as an internal standard. The proposed method is rapid, accurate and selective; it may be used for the quantitative analysis of meloxicam from raw materials, in bulk drugs and other dosage formulations.
Publication Details
Page(s) 58-62
DOI DOI not available
Published Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 18, Issue: 1, Year: 2005
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