Abstract:
Dopamine is an important biomolecule for human bodies. When the level of Dopamine in human body is abnormal then they causes various diseases such as Alzheimer's disease, epilepsy, addiction, obesity, euphoria, neuroblastoma, and pheochromocytoma. In the present study wede signed Cobalt doped Hydroxyapatite based biosensor for the detection of Dopamine in euphoria patient's blood serum sample. The characterization of synthesized Cobalt doped Hydroxyapatite nanocomposite was done by various techniques such as FTIR, SEM and XRD. The ionic Cobalt doped Hydroxyapatite nanocomposite shows intrinsic peroxidase-like activity, and in the presence of H2O2the oxidation of the peroxidase substrate 3, 3', 5, 5'-tetramethylbenzidine could be catalyze into a greenish colored product. Based on this phenomenon, for the colorimetric determination of Dopamine in human serum sample Cobalt doped Hydroxyapatite nanocomposite based sensor was developed. The optimization of the proposed sensor was done with different parameters such as (amount of doping agent, H2O2, pH, TMB, time and dopamine concentration) to get the best results. The proposed sensor provide a sensitive and simple effect for dopamine detection using TMB,H2O2 and Cobalt doped Hydroxyapatite nanocomposite with a linear range from 8 x10-9-3.68x10-7 M with low limit of detection 1.6x10-8 M, low limit of quantification 5.6x10-8 M and R2 0.9994. For the colorimetric detection of dopamine the proposed sensor gave a short response time at 4 min, room temperature and pH 4. Moreover, for the detection of dopamine in human serum samples the proposed method can be successfully applied.
Page(s):
423-423
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
hydrogen peroxide
,
Dopamine
,
peroxidase mimic
,
Colorimetric sensor
,
Cobalt doped Hydroxyapatite nanocomposite