The new lichen species Buellia pakistanica is described from Margalla Hills, Pakistan. It is characterized by larger weakly pruinose areoles (0.5-2 mm wide), frequent apothecia with diameter ranging from 0.5-1.4 mm, taller hypothecium (200-350 µm) and the absence of specific substances. ITS based phylogenetic analysis placed it sister to B. pruinocalcarea. The new species found in scrub forest with temperate climate growing on limestone rocks of Margalla Hills. Morphoanatomical description, chemical characterization of our taxon and a key of species of genus known from Pakistan are provided.