The PgCadh gene in pink bollworm (Pectinophora gossypiella) is involved in resistance suppression against Cry1Ac in Pakistan | [Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23 • 2023]

Author(s):

1. Jam Nazeer Ahmad: Integrated Genomic, Developmental and Biotechnology Laboratory, Dept. of Entomology; University of Agriculture Faisalabad, Pakistan; Plant Stress Physiology and Molecular Biology Lab, Dept. of Botany, University of Agriculture Faisalabad, Pakistan

2. Dilawar Majeed: Integrated Genomic, Developmental and Biotechnology Laboratory, Dept. of Entomology, University of Agriculture Faisalabad, Pakistan

3. Samina Tanwir: Integrated Genomic, Developmental and Biotechnology Laboratory, Dept. of Entomology; University of Agriculture Faisalabad, Pakistan; Plant Stress Physiology and Molecular Biology Lab, Dept. of Botany, University of Agriculture Faisalabad, Pakistan

Abstract:

Gene silencing through RNA interference in different insect pests has been targeted from previous few years. It has been exploited to inhibit growth of insect, induce mortality, avoids resistance evolution and increases the chances of insect susceptibility to Cry1Ac and pesticides. Here, two field collected populations of Pectinophora gossypiella (Multan and Faisalabad) and one susceptible and other resistant laboratory population was used for investigation. All populations were examined for PgCad1 gene expression that is involved in Bt resistance against P. gossypiella. The double stranded RNA (dsRNA) specific forPgCad1 gene in Pectinophora gossypiella was developed and employed through micro-injection using different concentration (1-3 ul/sample) to knockdown it in resistant population. The results showed that expression of PgCadh1 was up regulated in Multan, and laboratory reared resistant population as compared to Faisalabad and laboratory reared susceptible population. Down regulation of PgCad1 gene was observed in 3rd instar larvae upon application of PgCad1-dsRNA RT-PCR and real time PCR. Increased mortality was observed in 3rd instar larvae treated with dsRNA as compared to control insects treated with water and dsGFP. Present study showed that dsRNA can cause severe effect on the P. gossypiella and persuade RNA interference. The findings also confirmed that RNAi application is appropriate tool to cause mortality and to reduce the evolution of Cry1Ac resistance in P. gossypiella.

Page(s): 154-154

DOI: DOI not available

Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023

Keywords:

Cry1Ac , gene silencing , Pectinophora gossypiella , dsRNA , PgCad1 , RNAi

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