A checklist of macrofungi of Pakistan published/cited in literature after 1997 is given. More than two hundred published articles were reviewed. In total 338 macrofungal species belonging to Ascomycetes and Basidiomycetes reported from Pakistan are given. Most of the species were collected from the Himalayan temperate forest of Pakistan which is one of the biodiversity hotspot in the world. Lichens are not included in this list.