Standrization of phytase and biomass production in submerged fermentation with aspergillus flavus Phy190 isolated from soil | [Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23 • 2023]

Author(s):

1. Zahid Manzoor: Department of Parasitology and Microbiology,,

2. Aftab Ahmad Anjum: Institute of Microbiology, University of Veterinary & Animal Sciences, Lahore, Pakistan

3. Syeda Maryam Hussain: Department of Livestock Production and Management

4. Muhammad Zishan Ahmad: Department of Livestock Production and Management

5. Murtaz Ul Hassan: Department of Parasitology and Microbiology

6. Muhammad Kamran: Department of Parasitology and Microbiology

7. Muhammad Shoaib: Department of Parasitology and Microbiology

8. Aayesha Riaz: Department of Veterinary Pathology

9. Zaib Ur Rehman: Department of Poultry Science

10. Ayesha Qaisar: Department of Zoology, Wildlife and Fisheries, PMASAAU, Rawalpindi, Pakistan

11. Ali Ahmad: Microbiology Section, Department of Pathobiology, Narowal Campus, University of Veterinary & Animal Sciences, Lahore, Pakistan

Abstract:

Phytase is a type of enzyme categorized under the phosphatase group that facilitates the breakdown of phytic acid, leading to the liberation of inorganic phosphorus that can be utilized by the host. In this study we standardized the production of phytase and biomass (estimated by the dry weight content g/L) by submerged fermentation with Aspergillus flavus PHY190 which was isolated from soil. Experimental assays were performed under diverse conditions of pH (3.0 to 6.0) and temperature (20 to 45oC), and the influence of these fluctuating conditions on the responses was studied through one variable approach. Outcome of temperature, pH, substrate type and concentration were observed using submerged fermentation. The maximum production of phytase was achieved when using pH 5.0, temperature 300 C and 4% oat bran as substrate. Phytase activity was increased in up to 3.68- fold and the biomass matter was increased in up to 3.78-fold. Bye-products of Agricultural industry such as oat bran can be used alternative for cost efficient phytase production method under optimized conditions. One-way Analysis of Variance (ANOVA) and Post-Hoc Multiple comparison test (Duncan) was performed to compare the differences between means using SPSS. Therefore, these results are of interest as they contribute for the development of an industrial process for phytase production with eminent yields by submerged fermentation.

Page(s): 118-118

DOI: DOI not available

Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023

Keywords:

Aspergillus flavus , Oat bran , phytase , Submerged fermentation

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