Modified picrate method for determination of cyanide in blood</s | [Pakistan Journal of Pharmaceutical Sciences • 2011]

Author(s):

1. Muhammad Avais: Department of Clinical Medicine and Surgery, University of Veterinary & Animal Sciences, Lahore, Pakistan

2. Muhammad Sarwar Khan: Department of Clinical Medicine and Surgery, University of Veterinary & Animal Sciences, Lahore, Pakistan

3. Muhammad Arif Khan: Department of Clinical Medicine and Surgery, University of Veterinary & Animal Sciences, Lahore, Pakistan

4. Kamran Ashraf: Department of Parasitology, University of Veterinary & Animal Sciences, Lahore, Pakistan

5. Amar Nasir: Department of Clinical Medicine and Surgery, University of Veterinary & Animal Sciences, Lahore, Pakistan

6. Masood Rabbani: University Diagnostic Laboratory, University of Veterinary & Animal Sciences, Lahore, Pakistan

7. Abu Saeed Hashmi: Institute of Biochemistry and Biotechnology, University of Veterinary & Animal Sciences, Lahore, Pakistan

Abstract:

Cyanide (CNÃ) is widely distributed in the ecosystem and has been associated with toxic effects in humans and animals. Most outbreaks of CNÃ poisoning in animals result from ingestion of plants containing cyanogenic glycosides. Various analytical techniques for estimating cyanide in blood are available. A simple picrate method was developed to determine blood CNÃ in goats. This assay is a modification of commonly available methods using picrate paper and those using Conway diffusion cells. Cyanide in blood was measured during and after IV administration of KCN at 0.6 mg/min for 1 h. Blood CNÃ levels in rabbits were determined after oral administration of KCN for 10, 20, 30 and 40 days. The CNÃ concentration in blood of goats was time-dependent and continued rising during infusion followed by gradual decline after infusion stopped. A calibration curve set by dissolving various concentrations of KCN in distilled water showed a linear relationship between CNÏ concentration and absorbance (R=0.995) ranging from 0.3-120 mg CNÏ/L. Blood CNÏ levels in rabbits showed time-dependent increase with maximum concentration (1.34 mg/L) at 40 days. This is a simple and inexpensive tool for the determination of blood CNÏ in the laboratory and under field conditions as well.

Page(s): 149-153

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 24, Issue: 2, Year: 2011

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