Ameliorating effect of sesamin on insulin resistance of hepatic L02 cells induced by high glucose/high insulin | [Pakistan Journal of Pharmaceutical Sciences • 2019]

Author(s):

1. Wang Mengxi: College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/ Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics,Nanjing,China

2. Liu Mo: College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/ Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics,Nanjing,China

3. Zhou Renjie: School of Biology and food engineering, Hefei University of Technology,Hefei,China

4. Wu Jing: School of Biology and food engineering, Hefei University of Technology,Hefei,China

5. Chen Dongya: Jiangsu Center for Disease Control and Prevention,Nanjing,China

6. Lei Hong: College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/ Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics,Nanjing,China

Abstract:

Sesamin (SES) has the ameliorating effect on L02 hepatocyte model of insulin resistance induced by high glucose and high insulin, based on insulin receptor signaling pathway IRS/PI3K/Akt. Treatment with SES (200, 100µg/ml) increased glucose consumption, glucose uptake and the intracellular glycogen synthesis of L02 hepatocyte model of insulin resistance significantly. Moreover, treatment with SES promoted the gene and protein expression levels of insulin receptor (InsR) and the post-receptor associated proteins, such as insulin receptor substrate 1 (IRS1), insulin receptor substrate 2 (IRS2), PI3K (phosphatidylinositol 3-kinase), GLUT4 (glucose transporter 4) significantly, which were determined by RT-PCR and immunoblot analysis. In conclusion, SES has the ameliorating effect on L02 hepatocyte model of insulin resistance induced by high glucose/high insulin, which might be related to its effect on promoting expression of insulin receptor and its associated proteins of IRS-PI3K-Akt passway, and thus promoting insulin sensitivity.

Page(s): 2733-2739

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 32, Issue: 6, Year: 2019

Keywords:

insulin resistance , Sesamin SES , insulin receptor , L02 hepatocytes , IRSPI3KAkt signaling pathway

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