A simple method for microtuber production in Dioscorea opposita using single nodal segments. | [Pakistan Journal of Botany • 2015]

Author(s):

1. Mingjun Li: College of Life Sciences, Henan Normal University, Xinxiang 453007, China;Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs, University of Henan Province, Xinxiang 453007, China;Engineering Laboratory of Green Medicinal Material Biotechnology, Henan Province, Xinxiang, China

2. Junhua Li: College of Life Sciences, Henan Normal University, Xinxiang 453007, China;Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs, University of Henan Province, Xinxiang 453007, China;Engineering Laboratory of Green Medicinal Material Biotechnology, Henan Province, Xinxiang, China

3. Yipeng Wang: College of Life Sciences, Henan Normal University, Xinxiang, China

4. Wen Liu: College of Life Sciences, Henan Normal University, Xinxiang, China

5. Xiaobo Guo: College of Life Sciences, Henan Normal University, Xinxiang, China

6. Shujie Li: College of Life Sciences, Henan Normal University, Xinxiang, China

7. Linlin Han: College of Life Sciences, Henan Normal University, Xinxiang, China

8. Zhihui Song: College of Life Sciences, Henan Normal University, Xinxiang, China

9. Xiting Zhao: College of Life Sciences, Henan Normal University, Xinxiang 453007, China;Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs, University of Henan Province, Xinxiang 453007, China;Engineering Laboratory of Green Medicinal Material Biotechnology, Henan Province, Xinxiang, China

10. Qingxiang Yang: College of Life Sciences, Henan Normal University, Xinxiang 453007, China;Key Laboratory for Microorganisms and Functional Molecules, University of Henan Province, Xinxiang, China

Abstract:

Dioscorea opposita Thunb. (Chinese yam) is an important tuber crop in East Asia because of its dual benefits edible and medicinal properties. Microtubers may provide a feasible alternative to in-vitro-grown plantlets as a means of micropropagation and a way to exchange healthy planting material. In this study, we have developed a simplified culture method for In vitro production of microtubers from D. opposita cv. ‘Tiegun’. In this method, microtubers formed in 98% of the internodes of single nodal segments after four weeks of dark-incubation when cultured in MS medium supplemented with 60 g sucrose l-1 with shaking. Anatomical observations strongly supported the process of tuberization. We also found that 66% of the microtubers produced In vitro sprouted two months after transfer to vermiculite. The protocol presented here provides a simple model for studying the physiological, biochemical, and molecular mechanisms of tuberization in D. opposita, and shows good potential for large-scale production of microtubers as well.

Page(s): 665-668

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 47, Issue: 2, Year: 2015

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