Abstract:
Large-scale use and misuse of antibacterial agents for infection prevention in aquaculture has led to concern about promotion of antibiotic resistance among bacteria of clinical significance to human disease. The objective of the present study was to assess the presence of antibiotic-resistant Enterobacteriaceae in cyprinid fish spp., i.e., Ctenopharyngodon idella, Labeo rohita, Hypophthalmichthys molitrix, Catla, Cirrhinus mrigala & Cyprinus carpio etc., collected from various fish hatcheries in Khyber Pakhtunkhwa. Diseased fishes were transported live in well-aerated water bags to the laboratory of Fisheries and Aquaculture, Department of Zoology KUST. The fishes were dissected & muscle, skin, liver, gills & intestine tissue were removed. The samples were subjected to serial dilution followed by culture on general media and differential media. Later on, gram Staining, microscopy & biochemical tests were performed for species identification. Analysis of the data revealed the presence of several members of Enterobacteriaceae viz Klebsiella, Enterobacter, Serratia, Escherichia coli, Proteus spp., Shigella & Salmonella. After the identification of Bacteria, the antimicrobial resistance of the isolates was assessed via Kirby Bauer's essay against selected antibiotics of ß-lactam ring viz Penicillin (Penicillin (P), Amoxicillin (AML), Oxacillin (OX)), Carbapenems (Imipenem (IPM)), Monobactams (Aztreonam (ATM)), & Cephalosporins (Ampicillin (AMP), Ceftazidime (CAZ), Ceftriaxone (CRO), Cephradine (CE), Cefoxitin (FOX), Cefepime (FEP)). Results of Kirby Bauer's essay demonstrated that the bacteria exhibited significant resistance to traditional antibiotics which is understandable because these antibiotics are being used for so long and their multiple exposures to bacteria enabled bacteria to develop resistance against them. The 3rd & 4th generations of ß-lactam antibiotics partially inhibited the growth of bacteria. The current study concludes that the indiscriminate use of antibiotics in aquaculture should be under strict regulation as the resistant bacterial isolates originated from fish may lead to potentially harmful effects on their human consumers when transferred via handling & food chain.
Page(s):
50-50
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Life Sciences (ICLS-23) 11-12 May 22-23, Volume: 0, Issue: 0, Year: 2023
Keywords:
antimicrobial
,
Enterobacteriaceae
,
ßlactam ring
,
Penicillins
,
Cyprinid