New insights on distribution and molecular characterization of sugarcane bacterial diseases in Pakistan | [First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book) • 2022]

Author(s):

1. Shumyle Tasaddaq: Department of Plant Pathology, PMAS Arid Agriculture University,Rawalpindi,Pakistan

2. Muhammad Usman Raja: Department of Plant Pathology, PMAS Arid Agriculture University,Rawalpindi,Pakistan

3. Tariq Mukhtar: Department of Plant Pathology, PMAS Arid Agriculture University,Rawalpindi,Pakistan

4. Muhammad Zakria: Crop Disease Research Institute, National Agriculture Research Centre,Islamabad,Pakistan

5. Muhammad Asad Farooq: Crop Disease Research Institute, National Agriculture Research Centre,Islamabad,Pakistan

Abstract:

-Phytobacteria are emerging as a threat to sustainable production of sugarcane (Saccharum officinarum L.) which considered as the second most important cash crop in Pakistan. During this study, major sugarcane growing areas of KPK, Punjab and Sindh provinces were surveyed in year 2020-21. Based on field symptomology, three bacterial diseases viz., red stripe, leaf scald and Stewart bacterial wilt were found in Punjab while in Sindh only leaf scald and red stripe were observed. The putative bacterial isolates initially confirmed as Xanthomonas albilineans (Xa), Pantoea stewartii subsp. stewartii (Pss) Acidovorax avenae subsp. avenae (Aaa) through morpho-biological assays. Bacteria showed maximum sensitivity against tetracycline in disc diffusion method. However, bacteria found insensitive against copper sulphate and grow at temperature range between 10 to 40oC. PCR test was used for the molecular confirmation of these bacteria. Xanthomonas albilineans was confirmed by specific primers XaF/XaR on which it showed band at 600 bp while Pantoea stewartii subsp. stewartii and Acidovorax avenae subsp. avenae gave clear bands at 1500bp with universal primers B16SF/B16SR and P0f/P6r respectively. Both Pss and Aaa were sequenced through sanger sequencing method by Macrogen company Korea. After blasting, our sequenced result of Pantoea stewartii subsp. stewartii and Acidovorax avenae subsp. avenae showed similarity with Chinese, Malaysian and USA isolates (MW015765, MF351732 for Pss and KU948662, CP028300 for Aaa respectively). Furthermore, these sequences were submitted to GenBank (NCBI) with the accession number SUB11720155 (ON965180, ON965181) for Pss and SUB11952181 (OP267574, OP267575) for Aaa. This pilot study provided insights regarding prevalence of phytobacteria in sugarcane areas which can be used in future sugarcane germplasm disease screening program.

Page(s): 0-0

DOI: DOI not available

Published: Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022

Keywords:

Sugarcane , Sugarcane , Acidovorax avenae subspavenae , Xanthomonas albilineans , Pantoea stewartii subsp stewartii

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