Xiao-Gao-Jiang-Zhuo-containing serum inhibits adipogenesis through SIRT1-IGF-1 crosstalk in 3T3-L1 preadipocytes | Pakistan Journal of Pharmaceutical Sciences 2023

Author(s)

Liang Chen Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences,Beijing,China

Yu Han Hospital Administration Office, China Academy of Chinese Medical Sciences,Beijing,China

Jing Li Department of Endocrinology, Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University,Urumqi,China

Chunpeng Feng Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences,Beijing,China

Chen Chen Department of Endocrinology, Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University,Urumqi,China

Ting Ye Department of Endocrinology, Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University,Urumqi,China

Abstract

PPAR?, CEBP/a, and SREBP1C are the major transcriptional factors participating in adipogenesis and lipogenesis. SIRT1 and IGF-1 signaling pathways are important pathways involved in body endocrine and metabolism. Our unique Chinese herbal medicine Xiao-Gao-Jiang-Zhuo (XGJZ) has a remarkable clinical effect on obesity. However, the molecular basis remains unknown. XGJZ-containing serum was treated in the incubation of 3T3-L1 preadipocytes to observe its function in the 3T3-L1 cell differentiation. Oil Red O staining was used to monitor the lipid droplets accumulated after 8 days of incubation. RT-qPCR and western blotting were used to investigate the regulatory effects of XGJZ-containing serum on adipogenesis-related factors. The protein levels of main molecules in SIRT1 and IGF-1 signaling pathways were also detected by western blotting. XGJZ-containing serum notably suppressed the lipid accumulation in differentiated adipocytes through SIRT1/IGF-1 pathway. XGJZ-containing serum activated the SIRT1/IGF-1 pathway and reduced the expression levels of PPAR?, CEBP/a, and SREBP1C through this pathway. Additionally, XGJZ-containing serum enhanced the phosphorylation of ATGL and HSL and then induced lipolysis. XGJZ-containing serum has inhibitory effects on adipogenesis in 3T3-L1 preadipocytes through SIRT1/IGF-1 signaling pathway. Our study affirmed the effect of XGJZ-containing serum in the treatment of obesity. It provides a basis for the mechanism of obesity.

Publication Details

Page(s) 773-782

DOI 10.36721/PJPS.2023.36.3.REG.773-782.1

Published Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 36, Issue: 3, Year: 2023

Keywords

Obesity Lipid metabolism XGJZ adipogenesis SIRT1 IGF1

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