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A genetic Linkage Map of Phalaenopsis-based on AFLP Markers and the “two-way Pseudo-testcross” Mapping Strategy.
Author(s)
Feixiong Liao College of Forestry and Landscape Archtecture, South China Agricultural University, Guangzhou 510642, China
Shenping Xu Institute of Bioengineering, Zhengzhou Normal University, Zhengzhou 450044, China; College of Forestry and Landscape Archtecture, South China Agricultural University, Guangzhou 510642, China
Abstract
Phalaenopsis are grown widely as cut-flowers and potted plants in the floriculture trade. Here, we report the genetic linkage maps for Phalaenopsis, which were developed by genotyping 88 F1 progenies from a cross between Phalaenopsis '462' (male) and Phalaenopsis '20' (female) with amplification fragment length polymorphisms (AFLP) in a “two-way pseudotestcross” mapping strategy. For a total of 416 polymorphic loci showing Mendelian segregation were generated from 71 AFLP primer combinations. The data were analyzed using JoinMap 3.0 to construct the parent-specific linkage maps. The paternal map consisted of 15 linkage groups and 122 AFLP makers, covering 820.3 cM with a mean distance of 6.7 cM between adjacent markers. The maternal map consisted of 14 linkage groups with 175 loci, resulting in a total genetic distance of 878.3 cM and an average genetic distance of 5.0 cM between adjacent markers. Yet, as far as we know, this is the first study to construct the linkage map of Phalaenopsis. The two maps will serve as the frameworks for mapping of horticultural quantitative trait locis (QTLs) and furnish reference information for the application of future marker-assisted selection in Phalaenopsis breeding.
Publication Details
Page(s) 551-557
DOI 10.17957/IJAB/15.0333
Published Journal: International Journal of Agriculture and Biology, Volume: 19, Issue: 3, Year: 2017
Keywords
Keywords are not available for this article.
References
Abe , H.,M.Nakano,A.Nakatsuka,M.Nakayama,M.Koshioka,M.Yamagishi, 2002.Genetic analysis of floral anthocyanin pigmentation traits in Asiatic hybrid lily using molecular linkage maps,Theor. Appl. Genet 8 1175 -1182
Been , C.,A.Na,J.Kim,H.Y.Kim, 2002.Random amplified polymorphic DNA (RAPD) for genetic analysis of Phalaenopsis species,Hortic. Environ. Biotechnol. 4 387 -391
Behrend , A.,T.Borchert,M.Spiller,A.Hohe, 2013.AFLP-based genetic mapping of the “bud-flowering” trait in heather (Calluna vulgaris),BMC Genet 1 1 -11
Bonnema , G.,P.V.D.Berg,P.Lindhout, 2002.AFLPs mark different genomic regions compared with RFLPs: a case study in tomato,Genome 2 217 -221
Cai , J.,X.Liu,K.Vanneste,S.Proost,W.C.Tsai,K.W.Liu,L.J.Chen,Y.He,Q.Xu,C.Bian,Z.Zheng,F.Sun,Y.Y.Hsiao,Z.J.Pan,C.C.Hsu,Y.P.Yang,Y.C.Hsu,Y.C.Chuang,A.Dievart,J.F.Dufayard,X.Xu,J.Y.Wang,J.Wang,X.J.Xiao,X.M.Zhao,R.Du,G.Q.Zhang,M.Wang,Y.Y.Su,G.C.Xie,G.H.Liu,L.Q.Li,L.Q.Huang,Y.B.Luo,H.H.Chen,Y.Van de Peer,Z.J.Liu, 2015.The genome sequence of the orchid Phalaenopsis equestris,Nat. Genet 1 65 -72
Capo-Chichi , L., C.M.,Morton and D.B.,Weaver, 2004.An intraspecific genetic map of velvetbean (Mucuna sp.) based on AFLP markers,Theor. Appl. Genet 5 814 -821
Chang , Y.,L.A.Blischak,R.E.Veilleux and M.J. Iqbal, 2010.Effect of temperature on gametophytic selection in a Phalaenopsis F1 population,Euphytica 2 251 -261
Chang , Y.,R.E.Veilleux and M.J. Iqbal, 2009.Analysis of genetic variability among Phalaenopsis species and hybrids using amplified fragment length polymorphism,J. Amer. Soc. Hortic. Sci. 1 58 -66
Chen , D.,L.Chen, 2010.The first intraspecific genetic linkage maps of wintersweet [Chimonanthus praecox (L.) Link] based on AFLP and ISSR markers,Sci. Hortic 1 88 -94
Chen , M.,F.Feng,X.Sui, 2013.Genetic linkage maps of Pinus koraiensis Sieb,et Zucc. based on AFLP markers. Afr. J. Biotechnol 35 5659 -5664
Christenson , E.A., 2001.,Phalaenopsis. a Monograph 68 -70
Darvasi , A.,A.Weinreb,V.Minke,J.I.Weller,M.Soller, 1993.Detecting marker-QTL linkage and estimating QTL gene effect and map location using a saturated genetic map,Genetics 3 943 -951
De la Rosa , R.,A.Angiolillo,C.Guerrero,M.Pellegrini,L.Rallo,G.Besnard,A.Berville,A.Martin,L.Baldoni, 2003.A first linkage map of olive (Olea europaea L.) cultivars using RAPD, AFLP, RFLP and SSR markers,Theor. Appl. Genet 7 1273 -1282
Dunemann , F.,R.Kahnau,I. Stange, 1999.Analysis of complex leaf and flower characters in Rhododendron using a molecular linkage map,Theor. Appl. Genet 1146 -1155
Gawenda , I.,A.Schröder-Lorenz,T.Debener, 2012.Markers for ornamental traits in Phalaenopsis orchids. population structure, linkage disequilibrium and association mapping,Mol. Breed 1 305 -316
Grattapaglia , D.,R.Sederoff, 1994.Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers,Genetics 4 1121 -1137
Haanstra , J., C.,Wye , H.,Verbakel , F.,Meijer-Dekens , P. Van den Berg, P. Odinot, A.W. Van,Heusden , S.,Tanksley , P.,Lindhout and J.,Peleman, 1999.An integrated high-density RFLP-AFLP map of tomato based on two Lycopersicon esculentum× L. pennellii F2 populations,Theor. Appl. Genet 254 -271
Hackett , C.A.,L.B.Broadfoot, 2003.Effects of genotyping errors, missing values and segregation distortion in molecular marker data on the construction of linkage maps,Heredity 1 33 -38
Han , T.,M. de Jeu and E.,Jacobsen, 2002.The construction of a linkage map of Alstroemeria aurea by AFLP markers,Euphytica 2 153 -164
He , D.,Y.Liu,M.Cai,H.Pan,Q.Zhang, 2014.The first genetic linkage map of crape myrtle (Lagerstroemia) based on amplification fragment length polymorphisms and simple sequence repeats markers,Plant 1 138 -144
Hibrand-Saint Oyant , L.,L.Crespel,S.Rajapakse,L.Zhang,F.Foucher, 2008.Genetic linkage maps of rose constructed with new microsatellite markers and locating QTL controlling flowering traits,Tree. Genet. Genomes 1 11 -23
Hong , Y.,X.Liang,X.Chen,G.Zhou,S.Li,S.Wen, 2008.Construction of Genetic Linkage Map Based on SSR Markers in Peanut (Arachis hypogaea L.),Agric. Sci. China 8 915 -921
Hsu , T.,W.Tsai,D.Wang,S.Lin,Y.Hsiao,W.Chen,H.Chen, 2008.and its somaclonal variant,Plant Sci. 3 415 -422
Kao , Y., S.,Chang , T.,Lin , C.,Hsieh , Y.,Chen , W.,Chen and C.,Chen, 2001.Differential accumulation of heterochromatin as a cause for karyotype variation in Phalaenopsis orchids, 3 387 -395
Kenis , K.,J.Keulemans, 2005.Genetic linkage maps of two apple cultivars based on AFLP and microsatellite markers,Mol. Breed 2 205 -219
Konishi , T.,K.Abe,S.Matsuura,Y.Yano, 1990.Distorted segregation of the esterase isozyme genotypes in barley (Hordeum vulgare L.),Jpn. J. Genet 6 411 -416
Kosambi , D.D., 1943.The estimation of map distances from recombination values,Ann. Hum. Genet 1 172 -175
Kriegner , A.,J.C.Cervantes,K.Burg,R.O.Mwanga,D.Zhang, 2003.A genetic linkage map of sweetpotato [Ipomoea batatas (L.) Lam.] based on AFLP markers,Mol. Breed 3 169 -185
Ky , C.,P.Barre,M.Lorieux,P.Trouslot,S.Akaffou,J.Louarn,A.Charrier,S.Hamon,M.Noirot, 2000.Interspecific genetic linkage map, segregation distortion and genetic conversion in coffee (Coffea sp,Theor. Appl. Genet 4 669 -676
Lee , M., 1995.DNA markers and plant breeding programs,Adv. Agron. 55 265 -344
Liao , L.,I.Pan,Y.Chan,Y.Hsu,W.Chen,M.Chan, 2004.Transgene silencing in Phalaenopsis expressing the coat protein of Cymbidium Mosaic Virus is a manifestation of RNA-mediated resistance,Mol. Breed 3 229 -242
Mignouna , H.,R.Mank,T.Ellis,N. Van Den Bosch,R.Asiedu,S.Ng,J.Peleman, 2002.A genetic linkage map of Guinea yam (Dioscorea rotundata Poir.) based on AFLP markers,Theor. Appl. Genet 5 716 -725
Murray , M.G.,W.F.Thompson, 1980.Rapid isolation of high molecular weight plant DNA,Nucleic. Acids Res 19 4321 -4326
Myburg , A.A.,A.R.Griffin,R.R.Sederoff,R.W.Whetten, 2003.Comparative genetic linkage maps of Eucalyptus grandis, Eucalyptus globulus and their F1 hybrid based on a double pseudobackcross mapping approach,Theor. Appl. Genet 6 1028 -1042
Ouédraogo , J.T.,B.S.Gowda,M.Jean,T.J.Close,J.D.Ehlers,A.E.Hall,A.G.Gillaspie,P.A.Roberts,A.M.Ismail,G.Bruening, 2002.An improved genetic linkage map for cowpea (Vigna unguiculata L.) combining AFLP, RFLP, RAPD, biochemical markers, and biological resistance traits,Genome 1 175 -188
Pradhan , A.,V.Gupta,A.Mukhopadhyay,N.Arumugam,Y.Sodhi,D.Pental, 2003.A high-density linkage map in Brassica juncea (Indian mustard) using AFLP and RFLP markers,Theor. Appl. Genet 4 607 -614
Sjahril , R.,D.P.Chin,R.S.Khan,S.Yamamura,Y.Amemiya,M.Mii, 2006.Transgenic Phalaenopsis plants with resistance to Erwinia carotovora produced by introducing wasabi defensin gene using Agrobacterium method,Plant 2 191 -194
Strommer , J.,J.Peters,J.Zethof,T.Gerats, 2002.AFLP maps of Petunia hybrida. building maps when markers cluster,Theor. Appl. Genet 1000 -1009
Turkeli , Y. and S.,Kafkas, 2013.First genetic linkage map in pistachio constructed using an interspecific cross between Pistacia vera L. and monoecious Pistacia atlantica Desf,Sci. Hortic 151 30 -37
Van Ooijen , J.W.,R.E.Voorrips, 2001.JoinMap 3.0 software, -
Voorrips , R. E., 2002.MapChart: software for the graphical presentation of linkage maps and QTLs, 1 77 -78
Vos , P.,R.Hogers,M.Bleeker,M.Reijans,M.Hornes,A.Friters,J.Pot,J.Paleman,M.Kuiper, 1995.AFLP: a new technique for DNA fingerprinting,Nucleic. Acids. Res. 21 4407 -4414
Wang , D.,Y.Li,L.Li,Y.Wei and Li,Z, 2014.The first genetic linkage map of Eucommia ulmoides,J. Genet 93 13 -20
Xue , D., S.,Feng , H.,Zhao , H.,Jiang , B.,Shen , N.,Shi , J.,Lu , J. Liu and H.,Wang, 2010.The linkage maps of Dendrobium species based on RAPD and SRAP markers,J. Genet 3 197 -204
Yan , Z.,C.Denneboom,A.Hattendorf,O.Dolstra,T.Debener,P.Stam and P.B. Visser, 2005.Construction of an integrated map of rose with AFLP, SSR, PK, RGA, RFLP, SCAR and morphological markers,Theor. Appl. Genet 4 766 -777
Zhang , F., S.,Chen , F.,Chen , W.,Fang and F.,Li, 2010.A preliminary genetic linkage map of chrysanthemum (Chrysanthemum morifolium) cultivars using RAPD, ISSR and AFLP markers,Sci. Hortic 3 422 -428
Zheng , Y.,H.Guo,G.Zang,J.Liu, 2013.Genetic linkage maps of centipedegrass [Eremochloa ophiuroides (Munro) Hack] based on sequence-related amplified polymorphism and expressed sequence tag-simple sequence repeat markers,Sci. Hortic 156 86 -92
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