Abstract:
We analyzed the epidemiological relationship of Clostridium (C.) perfringens isolated from feces and soil of horse farm using multi-locus sequence typing (MLST) and then compared it with standard strains registered in the National Center for Biotechnology Information. MLST results using MEGA 6.0 showed that total 13 clusters were formed in the phylogenetic tree of the housekeeping genes sequence of the standard strains, and the Korea Isolate Ju (KSJ) strains were classified into 8 types (cluster 4, cluster 5, cluster 6, cluster 7, cluster 8, cluster 11, cluster 12, and cluster 13). The KSJ strains were categorized into 4 groups. Each group had a high bootstrap value (>90%). These results for C. perfringens are considered to be helpful for performing epidemiological investigations and establishing prevention methods for diseases in the future.
Keywords:
Soil
,
Horse
,
Clostridium perfringens
,
multilocus sequence typing