Abattoir based sero-survey of mycobacterium avium subspecies paratuberculosis in bovines in district Faisalabad-Pakistan | [Pakistan Journal of Zoology • 2020]

Author(s):

1. Rais Ahmed: Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan; Department of Microbiology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan

2. Muhammad Khalid Mansoor: Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan; Department of Microbiology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan

3. Iftikhar Hussain: Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan

4. Muhammad Saqib: Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan

5. Muhammad Hammad Hussain: Animal Health Research Center, Ministry of Agriculture and Fisheries, Muscat, Oman

6. Amjad Islam Aqib: Department of Medicine, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan

7. Haleema Sadia: Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta, Pakistan

8. Javed Muhammad: Department of Microbiology, University of Swabi, Anbar, Pakistan

9. Asma Irshad: National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan

10. Kashif Prince: Department of Medicine, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan

11. Muhammad Zain Saleem: Department of Pathology, University of Veterinary and Animal Sciences, Lahore, Pakistan

12. Abdul Whab Manzoor: Veterinary Research Institute, Zarrar Shaheed Road, Lahore, Pakistan

Abstract:

Bovine paratuberculosis is a chronic disease of cattle and buffaloes, causing progressive weight loss, persistent diarrhea and finally death. Due to zoonotic nature of the disease, workers in slaughterhouses are at a high risk of infection. In this study, the status of paratuberculosis was assessed in a slaughterhouse located in district Faisalabad, Pakistan. A total of 455 blood samples were collected from slaughtered cattle and buffaloes at random and then tested by a commercially available ELISA kit for the detection of antibodies against Mycobacterium avium subsp. paratuberculosis (MAP). Data were analyzed using chi square for ELISA and odds ratio for finding association of species, gender, body weight, age and body condition score (BCS) with prevalence of antibodies against MAP. Seropositivity against MAP was significantly (P<0.05) detected. Male and female animals were equally susceptible to the disease (OR: 0.908, 95% CI= 0.6111-1.350). Age groups were not found associated with chances of being seropositive. Seropositivity was significantly (P<0.05) higher in animals under 300kg body weight. Chances of being seropositive were found more in animals belonging to BCS 1 (OR: 13.25, 95%CI=6.29-27.88) and BCS 3 (OR: 4.78, 95%CI=2.36-9.70) than BCS 4 and above. In conclusion, gender, body weight and body condition score of the animals are positively associated with the occurrence of the disease. These data will not only help in screening of animals but will also be instrumental in future for isolation of MAP to make vaccine against paratuberculosis using local isolates.

Page(s): 115-119

DOI: 10.17582/journal.pjz/2020.52.1.115.119

Published: Journal: Pakistan Journal of Zoology, Volume: 52, Issue: 1, Year: 2020

Keywords:

prevalence , Elisa , odds ratio , Bovine paratuberculosis , Cattle and buffalo

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