A highly efficient regeneration system for watermelon (Citrullus lanatus Thunb.). | Pakistan Journal of Botany 2013

Author(s)

Xuezheng Wang Horticulture College, Northeast Agricultural University, Harbin 150030, P.R. China

Limin Shang Horticulture College, Northeast Agricultural University, Harbin 150030, P.R. China

Feishi Luan Horticulture College, Northeast Agricultural University, Harbin 150030, P.R. China

Abstract

We developed a high frequency watermelon regeneration system using two inbred lines of watermelon (Citrullus lanatus), ‘W1-4’ and ‘W1-12’. Shoots were induced from cotyledonary nodes cultured on Murashige and Skoog (MS) basal medium solidified with agar (7.0 g/L) and containing various concentrations of cytokinin (6-benzyladenine) 6-BA) and auxin (indoleacetic acid) IAA). The highest rate of bud organogenesis was on MS medium containing 1.5 mg/L 6-BA + 0.2 mg/L IAA for ‘W1-4’ and on MS medium containing 1.0 mg/L 6-BA + 0.1 mg/L IAA for ‘W1-12’. The regeneration rate was higher in ‘W1-12’ than in ‘W1-4’. The best medium for shoot elongation in both inbred lines was MS containing 0.05 mg/L 6-BA. Regenerated plants showed the best rates of root formation on 1/2 MS containing 0.1 mg/L IAA. The rooted plants were carefully washed to remove all medium from the roots, and then transferred to soil in a greenhouse. The plants showed a 100% survival rate when transferred to soil. This highly efficient regeneration system will be useful for regenerating plants in genetic engineering applications, and is a useful tool for further genetic transformation studies on watermelon.

Publication Details

Page(s) 145-150

DOI DOI not available

Published Journal: Pakistan Journal of Botany, Volume: 45, Issue: 1, Year: 2013

Keywords

Keywords are not available for this article.

References

References are not available for this document.

Citations

Citations are not available for this document.