The effect of Phaseolus vulgaris L. Fixed Oil on the Behavioural Activity of Swiss Albino Mice. | [RADS Journal of Pharmacy & Pharmaceutical Sciences • 2018]

Author(s):

1. Sadia Ghousia Baig: Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan

2. Muhammad Mohtasheemul Hasan: Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan

3. Salman Ahmed: Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan

4. Syeda Anum Fatima: Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan

5. Salma: Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan

Abstract:

Background:Phaseolus vulgaris commonly known as kidney bean has been studied previously for pharmacological activities like antidiabetic, antioxidant, analgesic, anti-inflammatory, antifungal and antibacterial properties. Various extracts of the seeds have been researched except fixed oils. Objective: Therefore objective of the present study is to investigate the behavioural activity of fixed oil extracted from Phaseolus vulgaris seeds. Methodology: Hole board, light and dark , stationary rod and open field tests were used for behavioral evaluation. Four groups were made with seven mice in each : (1) Control = Normal saline 2ml/kg (2) Phaseolus vulgaris fixed oil = PVFO 2ml/kg (3) PVFO 4ml/kg (4) Diazepam (1mg/kg) as standard. All treatments were given orally, 30 minutes prior to the performance of tests. In hole board test PVFO 4ml/kg decreased the number of head poking (p=0.01). Results:In light and dark test PVFO in both doses decreased the time spent in light compartment (p=0.01). In open field test number of peripheral lines crossed were decreased (p=0.05) whereas in stationary rod test no effect was observed by any dose of PVFO. Conclusion:Results indicate that Phaseolus vulgaris fixed oil does not affect memory or learning in mice and may possess a dose dependent sedative hypnotic effect.

Page(s): 76-82

DOI: DOI not available

Published: Journal: RADS Journal of Pharmacy & Pharmaceutical Sciences, Volume: 6, Issue: 1, Year: 2018

Keywords:

hole board , Phasaeolus vulgaris seeds , open field , sedative

References:

[1] DoukkaliZ,TaghzoutiK,Houcine BouididaEL,NadjmouddineM,CherrahY, 2015.Alaoui K . Evaluation of anxiolytic activity of methanolic extract of Urtica urens in a mice model,Behav 11 1 -5

[2] DunlopBW,NemeroffCB ., 2007.The role of dopamine in the pathophysiology of depression, 64 327 -37

[3] FernandesMF,MutchDM,Leri F, 2017.The relationship between fatty acids and different depression-related brain regions, and their potential role as biomarkers of response to antidepressants, 9 298 -

[4] LakhwaniL,TongiaSK,PalVS,AgrawalRP,NyatiP,Phadnis P. Omega, 2007.-3 fatty acids have antidepressant activity in forced swimming test in wistar rats,Acta Pol Pharma 64 271 -6

[5] McNamaraRK,CarlsonSE, 2006.Role of omega-3 fatty acids in brain development and function: potential implications for the pathogenesis and prevention of psychopathology,Prostaglandins Leukot Essent Fatty Acids; 75 329 -49

[6] BhatiaHS,AgrawalR,SharmaS,HuoYX,YingZ,Gomez-Pinilla F. Omega, 2011.-3 fatty acid deficiency during brain maturation reduces neuronal and behavioral plasticity in adulthood,PLoS One 6 28451 -

[7] BeezholdBL,JohnstonCS,DaigleDR, 2010.Vegetarian diets are associated with healthy mood states: a cross-sectional study in seventh day adventist adults, 9 26 -

[8] BeezholdBL,Johnston CS, 2012.Restriction of meat, fish, and poultry in omnivores improves mood: a pilot randomized controlled trial,Nutr 11 9 -

[9] BazinetRP,LayeS ., 2014.Polyunsaturated fatty acids and their metabolites in brain function and disease,Nat. Rev. Neurosci; 15 771 -85

[10] GowRV,HibbelnJR, 2014.Omega-3 fatty acid and nutrient deficits in adverse neurodevelopment and childhood behaviors,Child Adolesc Psychiatr Clin N Am 23 555 -90

[11] HussainG,SchmittF,LoefflerJP,Gonzalez de Aguilar JL, 2013.Fatting the brain: A brief of recent research,Front. Cell. Neurosci; 7 144 -

[12] GrelaER,Gunter KD, 1995.Fatty acid composition and tocopherol content of some legume seeds,Animal Feed Sci. Technol 52 325 -31

[13] TakedaH,TsujiM,MatsumiyaT., 1998.Changes in headdipping behavior in the hole-board test reflect the anxiogenic and/or anxiolytic state in mice,Eur J Pharmacol.; 350 21 -9

[14] CrawleyJN,Goodwin FK, 1980.Preliminary report of a simple animal behaviour for the anxiolytic effects of benzodiazepines,Pharmacol Biochem Behav 13 167 -70

[15] WalshRN,CumminsRA ., 1976.The open-field test: a critical review,Psychological Bulletin 83 482 -504

[16] KishiokaA,FumiakiF,TamaeI,HirotakaK,HisashiM,ToshioI, 2009.A novel form of memory for auditory fear conditioning at a low intensity unconditioned stimulus,PLoS ONE 4 41 -57

[17] KishoreRN,AnjaneyuluN,Naga Ganesh M and Sravya N, 2012.Evaluation of anxiolytic, -

[18] 2012.activity of ethanolic extract of Foeniculum vulgare in mice model,Int J Pharmacy Pharmaceutical; 4 584 -

[19] LaderM,Morton S, 1991.Benzodiazepine problems, 86 823 -8

[20] PrutL,BelzungC ., .The open field as a paradigm to measure the effects of, -

[21] 2003.drugs on anxiety-like behaviors: a review,Eur J Pharmacol 463 3 -33

Citations

Citations are not available for this document.

Citations

Downloads

Views