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Transcriptome Analysis Reveals the Immune Response of Chicken Erythrocytes to Marek's Disease Virus Infection
Author(s):
1. Guan-bao Ning: College of Veterinary Medicine, Shanxi Agricultural University,Taigu,China
2. Sheng Niu: College of Veterinary Medicine, Shanxi Agricultural University,Taigu,China
3. Yue-jian Li: College of Veterinary Medicine, Shanxi Agricultural University, Taigu, China; Weinan Animal Health Supervision Institute, Weinan 714000, China
4. Xiao-xiao Lu: College of Veterinary Medicine, Shanxi Agricultural University, Taigu, China; Wenxian Forestry Bureau, Wenxian 454850, China
5. Shi-xiong Yang: College of Veterinary Medicine, Shanxi Agricultural University,Taigu 030801,China
6. Ali-Raza Jahejo: College of Veterinary Medicine, Shanxi Agricultural University,Taigu 030801,China
7. Ding Zhang: College of Veterinary Medicine, Shanxi Agricultural University,Taigu 030801,China
8. Wei-fang Hao: Taiyuan Center for Disease Control and Prevention,Taiyuan 030024,China
9. Wen-wei Gao: College of Veterinary Medicine, Shanxi Agricultural University,Taigu 030801,China
10. Yu-jun Zhao: College of Veterinary Medicine, Shanxi Agricultural University,Taigu 030801,China
11. Jian-hui Li: College of Veterinary Medicine, Shanxi Agricultural University,Taigu 030801,China
12. Fang Yan: College of Veterinary Medicine, Shanxi Agricultural University,Taigu 030801,China
13. Rong-kun Gao: College of Veterinary Medicine, Shanxi Agricultural University,Taigu 030801,China
14. Yu-hai Bi: College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801,China; CAS Key Laboratory of Pathogenic Microbiology and Immunology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences, Beijing 100101, China
15. Wen-xia Tian: College of Veterinary Medicine, Shanxi Agricultural University,Taigu 030801,China
16. Ling-xia Han: Unit of Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
Abstract:
Marek’s disease virus (MDV) is a highly cell-associated, oncogenic alpha herpesvirus that causes Marek’sdisease (MD) in chickens. Chicken erythrocytes are nucleated cells of myeloid origin which contributeto gases exchange, transportation and immunity. The objective of this study was to reveal the immuneresponses of MDV-infected erythrocytes of chicken by transcriptome analysis. Twenty specific pathogenfree(SPF) chickens were divided into two groups as control and treatment. The chickens of treatmentgroup were inoculated with MDV, and blood samples of two groups were obtained at 14 and 22 dayspost-infection (dpi) for RNA extraction. Transcriptome analysis was performed to explore the immuneresponses of chicken erythrocytes to MDV infection at 14 dpi and 22 dpi. In total, 129.6 million reads oftwo groups at two time points were mapped against the reference genome (Gallus gallus 5.0). A significantdifferential pattern of the immune response of chicken erythrocytes was identified by the GO and KEGGdatabases between the two phases of MDV infection. The results indicated that cytokine-cytokine receptorinteraction, toll-like receptor signaling pathway, mitogen-activated protein kinases (MAPK) signalingpathway, Janus kinase/signal transducers and activators of transcription (JAK-STAT) signaling pathwaywere involved in the response to MDV infection. The expression levels of all most differentially expressedgenes (DEGs) in these four pathways were upregulated significantly at 14 and 22 dpi, indicating that theimmune responses of erythrocytes were induced by MDV. This study may help to elucidate the molecularmechanism of chicken erythrocytes relate to immune responses in MDV infection.
Page(s): 2057-2069
DOI: 10.17582/journal.pjz/20191012061013
Published: Journal: Pakistan Journal of Zoology, Volume: 53, Issue: 6, Year: 2021
Keywords:
Immune response , Transcriptome
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